Water

Biotium Acridine Orange, 10mg/mL in water (AO)

Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies. Acridine orange has also been used for the detection of microorganisms in cerebrospinal fluid and other clinical specimens. Biotium offers a highly purified form of acridine orange while most other commercially available grades of AO are either in zinc chloride complex form or of low purity. The dye has an excitation/emission of 500/526 nm when bound to DNA and an excitation/emission of 460/650 nm when bound to RNA. Properties: Yellow solution; Store at 4°C, protect from light; Ex/Em = 500/526 nm (DNA), 460/650 nm (RNA); C17H20ClN3; MW:301.82; [65-61-2].