Benzoxazines

Organic heterocyclic compounds that consist of benzene fused with an oxazines; oxazines are a six-membered ring with four carbon atoms, one oxygen atom, and one nitrogen atom.

Chemical. CAS 1152-14-3. Formula C14H9NO4. MW 255.23. Resorufin acetate is a fluorogenic substrate for hydrolytic esterases and cellulases used for monitoring cytosolic aldehyde dehydrogenase ALDH1A1 esterase activity, chymotrypsin activity. Resorufin acetate has been used as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase GOD, without significant effects of ascorbic acid, uric acid, or bilirubin. It also has been used for the chromogenic and fluorescent detection of hydrazine, acyl protein thioesterase APT enzymes and perborate ions, and reveals a selective turn-on type chromogenic and fluorogenic signaling behavior based on the selective and efficient cleavage of acetate group. Upon enzymatic cleavage of the acetate group of resorufin acetate, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin lambdaex=571nm, lambdaem=585nm.