Cycloheximide (CAS 66-81-9) is a small molecule inhibitor that specifically disrupts protein biosynthesis in eukaryotic cells by interfering with translational elongation at the ribosomal level It is widely employed in biomedical research to transiently block protein synthesis in vitro facilitating the study of cellular processes dependent on active translation Due to its cytotoxicity teratogenic potential and capacity to induce DNA damage cycloheximide is exclusively utilized for experimental research applications and inappropriate for clinical therapeutic use