Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences Succinyl-Concanavalin A ly25MG
Succinyl-Concanavalin A ly25MG
Sigma Aldrich Fine Chemicals Biosciences KiCqStartR SYBRR Green qPC
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer stabilizers and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency sensitivity specificity and robust fluorescent signal using fast or conventional cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye an antibody-mediated Hot-Start Taq DNA polymerase dNPTs MgCl2 and proprietary buffers and stabilizers.
Sigma Aldrich Fine Chemicals Biosciences Ribonucleoside Triphosphat
Ribonucleoside Triphosphate Set consists of four separate vials of ATP CTP GTP and UTP. Each vial contains a 100 mM clear colorless SOLUTIONon of the lithium salt (pH 7).
Sigma Aldrich Fine Chemicals Biosciences Mf MilliporeR Membrane fil
Biologically inert mixtures of cellulose acetate and cellulose nitrate have made Mf-Millipore membrane filters one of the most widely used membranes in analytical and research applications. Mf-Millipore filters without Triton surfactant contain minimum amounts of wetting agent and have a lower water extractable content than standard Mf-Millipore filters.features Benefits - Versatile filter for biological and environmental monitoring applications - Available in a range of pore sizes colored black or white with or without a gridded surface - Compatible with ethylene oxide gamma irradiation and autoclave sterilization methods
Sigma Aldrich Fine Chemicals Biosciences Tyrodes Salts With sodium500ML
Tyrodes Salts With sodium500ML
Sigma Aldrich Fine Chemicals Biosciences Albumn-fluorescen soth250MG
Albumin makes up 60% of the plasma protein which is produced by the liver. It regulates colloid osmotic pressure. Albumin is implicated in the transportation distribution and metabolism of fatty acids thyroid hormones and drugs. It reduces weight gain blocks pulmonary edema decreases ascites and lowers tissue edema.
Sigma Aldrich Fine Chemicals Biosciences RNAzolR RT for processing
RNAzol RT is a quick and convenient reagent for use in the single-step isolation of total and small RNA frombiological samples ofhuman animal plant yeast bacterial and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA protein polysaccharides and other molecules. RNAzol(R) RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA containing all classes of RNA in a single fraction. This product a mixture of guanidine thiocyanate and phenol in a monophase SOLUTIONon effectively dissolves DNA RNA and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA proteins polysaccharides and other molecules which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol(R) RT is sufficient to isolate RN
Sigma Aldrich Fine Chemicals Biosciences WilmadR low pressure/vacuu1EA
Novel low pressure/vacuum tube for NMR and EPR spectroscopy. A 4X larger sealing surface eliminates leaks and greatly increases its lifetime when compared to traditional gas-tight tubes. Offers a convenient flame-free sealing SOLUTIONon for air sensitive or volatile liquid samples at any pressure from vacuum to pressure slightly above 1 bar.features Greaseless PTfE piston provides a 100% contamination-free seal. Axial symmetric design guarantees its application in spinning experiments.Economical. reliable CAUTION Due to the nature of glass Extreme Caution should be exercised when using at elevated or reduced pressures as performance of tubes under pressure or vacuum cannot be guaranteed. Adequate safety shielding should always be used when working in these conditions.
Sigma Aldrich Fine Chemicals Biosciences NorellR Select SeriesTM 3
The high-throughput NMR tubes are ideal for reducing salt effects when running buffered SOLUTIONons for bio-samples. They are made from ASTM Type 1 Class A borosilicate glass for reproducibility and durability and are specially designed for one time use for routine NMR.
Sigma Aldrich Fine Chemicals Biosciences fastStartTM High fidelity
The enzyme dNTPack comprises the fastStart High fidelity PCR System and a ready-to-use SOLUTIONon of PCR grade nucleotides. The fastStart High fidelity PCR System is a blend of fastStart Taq DNA Polymerase and a thermostable proofreading protein which is also chemically modified. This protein mediates proofreading activity but carries no polymerase activity. Both proteins are inactive below 75C and are activated by heating to 95C for two minutes. The fastStart(R) High fidelity PCR System is the ideal tool for all amplification experiments where a combination of high specificity sensitivity accuracy and yield is needed. Hot start protocols have improved specificity sensitivity and PCR yield. Heat-labile blocking groups on amino acid residues of fastStart Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically.
Sigma Aldrich Fine Chemicals Biosciences TRI ReagentR LS for proces
TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation ofRNA DNA and proteinfrom liquid samples of human animal plant yeast bacterial and viral origin. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA DNA and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes and many samples can be simultaneously extracted.TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase SOLUTIONon. When a biological sample is homogenized or lysed with it and chloroform or 1-bromo-3-chloropropane is added the mixture separates into 3 phases an aqueous phase containing RNA the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phas
Sigma Aldrich Fine Chemicals Biosciences Yeast Synthetic Drop-out M
YEAST Yeast Synthetic Drop-out M