Chromatography Columns and Cartridges
Waters Corp VanGuard Cartridge, C8, 3.5 μm, 2.1 mm x 5 mm
These VanGuard Cartridges protect analytical columns by removing particulate contamination from the mobile phase, extending their lifetime and performance. They are optimized for 2.1 mm and 3.0 mm I.D. columns with 3.5 μm sorbent particles.
- Chemistry: C8
- Separation mode: Reversed phase
- Particle substrate: Hybrid
- pH range: 1 to 12 pH
- Maximum pressure: 18000 psi (1240 Bar)
- Pore size: 130 Å
- Particle size: 3.5 μm
- Inner diameter: 2.1 mm
- Length: 5 mm
- Units per package: 3 pk
Waters Corp SEC Column, 250 Å, 2.5 μm, 7.8 mm ID, 300 mm Length, with MaxPeak Premier Protein SEC Guard
This SEC column provides reliable protein size variant separations by combining MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. It minimizes undesired secondary interactions, which reduces method development time and improves data confidence for complex biotherapeutics.
- Achieves reliable protein size variant separations from 10,000 to approximately 650,000 Daltons.
- Minimizes undesired secondary ionic or hydrophobic interactions.
- Reduces method development by enabling a “generic” approach for various samples.
- Increases analyte recovery, sensitivity, and reproducibility with MaxPeak High Performance Surfaces technology.
- Reduces column conditioning and passivation times.
- Allows for simpler mobile phases without complex additives.
- Lowers the cost per analysis by utilizing Premier SEC Guards.
Waters Corp Column, Peptide CSH C18, 1.7 μm, 2.1 x 50 mm
This column is optimized for peptide separations, delivering excellent peak shape for peptides using FA- or TFA-containing mobile phases. It is suitable for LC or LC-MS applications and enhances reproducibility, peak shape, and recovery for metal-sensitive analytes. An integrated, replaceable 5 mm pre-column protects the analytical column.
- Optimized for peptide separations, analysis, and mapping.
- Provides excellent peak shape for peptides with FA- or TFA-containing mobile phases.
- Enhances reproducibility, peak shape, and recovery for metal-sensitive analytes.
- Features a fully integrated and easily replaceable 5 mm pre-column to protect the analytical column.
Waters Corp Preparative Column, 2-Picolylamine (2-PIC), 5 μm, 19 × 150 mm
This OBD 19 × 150 mm Torus 5 μm Preparative Column, featuring 2-PIC chemistry, is designed for general use in separating acidic and basic compounds.
- Based on patent-pending two-stage functionalization of ethylene bridged hybrid (BEH) particles.
- Hydrophilic surface for controlled retention and minimized unwanted interactions.
- Provides individual selectivity and improved peak shape for acidic and basic compounds.
- Offers novel selectivity versus conventional 2-ethylpyridine (2-EP) phases.
- Enhanced retention for diverse applications.
- Suitable for method transfer and scale-up from analytical to preparative SFC.
- Robust for laboratory-scale purification, maintaining retention and selectivity over time.
- Patented OBD Technology contributes to excellent column lifetimes.
Waters Corp BioResolve SCX mAb Column, 3 μm, 2.1 mm, 100 mm
Waters BioResolve SCX mAb Column, 3 μm, 2.1 mm, 100 mm
Waters Corp Column, Premier BEH C18 AX VanGuard FIT, 2.5 μm, 2.1 x 150 mm
The Atlantis Premier BEH C18 AX Columns offer excellent retention for polar acidic analytes under reversed phase conditions. Their reversed phase/anion-exchange mixed-mode chemistry is stable from pH 2-10 and provides complementary selectivity, beneficial for methods development. The VanGuard FIT Column format is an all-in-one guard-column design that reduces dead volume and extends column lifetime.
- Excellent retention for polar acidic analytes.
- Mixed-mode chemistry (pH 2-10) provides complementary selectivity.
- Guard-column design reduces dead volume and extends column life.
- Prevents unwanted sample matrix and particle introduction.
- Extends analytical column operational lifetime.
- High-performance packing for biological fluid cleanup, temperature control.
- Maximum operating limits: 10 pH, 60 °C, 18,000 psi (1240 Bar).
Waters Corp ACQUITY UPLC Column, Peptide HSS T3, 1.8 μm, 1 mm X 100 mm
These Peptide HSS T3 Columns are engineered for separations requiring silica-based selectivities or enhanced retention of hydrophilic peptides. Each batch of HSS T3, 100Å material undergoes rigorous quality control testing with a tryptic digest of cytochrome c to ensure stringent performance. Optimized for low dispersion micro-flow systems, these 1 mm ID columns are particularly effective for LC-MS methods.
- Each batch of HSS T3, 100Å material is quality control tested with a gradient separation of a tryptic digest of cytochrome c and must pass stringent performance criteria.
- 1 mm ID columns are recommended for use with low dispersion micro-flow systems, especially for LC-MS methods.
- Suggested Use: Proteomics, Peptide Separations, Peptide Analysis, Micro-flow.
Waters Corp XBridge BEH C18 OBD Prep Column, 10 μm, 10 mm × 250 mm
As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1 to 12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed.
- Broad pH range (1 to 12) for method development.
- Trifunctionally bonded C18 ligand for universal HPLC separations.
- Superior low pH stability extends column life.
- Ultra-low column bleed improves detection sensitivity.
- Optimum Bed Density (OBD) design resists mechanical failure and pressure shocks.
- Increased productivity through faster lab-scale separations and seamless scaling.
- Longer column lifetimes reduce replacement frequency.
Waters Corp XBridge Premier Protein SEC Columns with MaxPeak Premier Protein SEC Guard, 250à , 2.5 µm, 7.8 x 150 mm, 1/pk
XBridge Premier Protein SEC Columns with MaxPeak Premier Protein SEC Guard, 250à , 2.5 µm, 7.8 x 150 mm, 1/pk
Waters Corp XBridge BEH C18 Column, 130 Å, 3.5 μm, 3 mm × 150 mm
This XBridge BEH C18 Column, 130 Å, 3.5 μm, 3 mm × 150 mm, includes an electronic eConnect column tag that contains unique column-specific information. These NFC-enabled, device-tagged columns were developed for automatic identification, tracking, and digital tracing of column use history. While eConnect columns can be used on any HPLC, their advantages are fully realized when used with a compatible system running Empower software.
- Compatible with any HPLC system.
- Chemistry: C18.
- Separation mode: Reversed phase.
- Particle substrate: Hybrid.
- pH range: 1 to 12.
- Maximum pressure: 6000 psi (415 bar).
- Endcapped.
- Low silanol activity.
- Particle shape: Spherical.
- Surface area: 185.
- System: HPLC.
- Particle technology: BEH.
- USP classification: L1.
- Application: Small molecule.
Waters Corp XTerra MS C18 Column, 125Å, 2.5 μm, 2.1 mm x 50 mm
This XTerra MS C18 Column is designed for high-performance liquid chromatography and features an eConnect Column Tag for digital integration and enhanced laboratory efficiency.
- Features an eConnect tag for digital integration.
- Stores unique product identifying information, automatically imported by Empower CDS.
- NFC enabled for web access and smart device support.
- Pre-equipped tag simplifies setup and reduces manual data entry.
- Tamper-proof design ensures compliance and auto-detection by Alliance iS.
- Tag design prevents interference in temperature-sensitive LC methods.
- Identical packing to untagged versions ensures consistent quality.
Waters Corp XSelect Premier Peptide CSH C18 Column, 130 Å, 2.5 μm, 2.1 x 100 mm
This column is specifically designed for peptide separations, featuring a low and carefully-defined concentration of positive charges that ensures excellent peak shape. It is ideal for LC and LC-MS applications due to minimal dependence on strong ion pairing agents. The 2.1 mm ID columns are recommended for low dispersion systems and are part of the MaxPeak Premier Class, delivering enhanced reproducibility, peak shape, and recovery for metal-sensitive analytes.
- First choice for peptide separations.
- Ensures excellent peak shape for peptides using FA- or TFA-containing mobile phases.
- Quality controlled with cytochrome c digest standards.
- Unique design includes an integrated, replaceable 5 mm pre-column to extend analytical column life.
- VanGuard FIT cartridge replacements use the same qualified sorbent.
- Suggested for peptide separations, analysis, mapping, and synthetic peptides.
Waters Corp Atlantis Column, 5 μm, 2.1 mm ID, 50 mm Length
The Atlantis Premier BEH C18 AX Columns offer excellent retention for polar acidic analytes under reversed-phase conditions. This mixed-mode chemistry provides complementary selectivity to traditional C18 phases and is stable across a pH range of 2 to 10, benefiting method development. It features MaxPeak High Performance Surface Technology to prevent signal intensity losses from unwanted analyte-surface interactions, ensuring data reproducibility for method validation and development. Ethylene-Bridged Hybrid (BEH) particle technology maximizes retention.
- Excellent retention for polar acidic analytes
- Reversed-phase/anion-exchange mixed-mode chemistry
- Stable from pH 2 to 10
- MaxPeak High Performance Surface Technology prevents signal loss
- Operates up to 18,000 psi
- Pore size: 95 Å
- Carbon load: 17%
- Maximum temperature: 60 °C
Waters Corp ACQUITY UPLC Peptide HSS T3 C18 Column
This column is designed for peptide separations and suitable for reversed-phase analysis of both hydrophilic and hydrophobic peptides.
- Equipped with MaxPeak HPS technology to prevent unwanted analyte-surface interactions.
- Provides ruggedness for reproducible data, attributed to the high-strength silica particle.
- Offers a wide, usable pH range.
- Aims to increase sample throughput and chromatographic resolution, reducing analysis time.
- Includes a Cytochrome C digestion standard for immediate benchmarking and ongoing monitoring.
- Recommended with the VanGuard pre-column for enhanced protection from fouling.
Waters Corp Spherisorb Cyano (CN) OBD Prep Column, 10 μm, 19 × 250 mm
This general purpose normal phase propyl cyano bonded ligand column on a silica substrate is conditioned and shipped with normal phase solvent. Its Optimum Bed Density (OBD) design ensures direct scalability and cyano base chemistry provides selectivity. Rugged silica particles reduce constant reproducibility needs.
- Cyano (CN) chemistry
- Normal-phase mode
- Silica particle substrate
- pH range 2 to 8
- Temperature limit 45°C
- Max pressure 6000 psi (415 Bar)
- Not endcapped
- High silanol activity
- Spherical particle shape
- 10 μm particle
- 80 Å pore
- OBD Prep Column format
- 220 surface area
- HPLC Preparative system
- LC/MS, LC techniques
- USP classification L10
- 19 mm inner diameter
- 250 mm length
- 3% carbon load