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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (23,376)
HPLC Guard Columns and Cartridges (3,063)
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271 – 285 of 21,365 results
271

MilliporeSigma™ Chromolith™ Performance Silica Normal Phase HPLC Column, Monolith, 4.6 mm x 100 mm

Column Format: Column; Pore Size: 130 Å (Mesopore Size), 2 μm (Macropore Size); Column Phase: Normal Phase

272

MilliporeSigma™ Chromolith™ WP 300 Protein A HPLC Column

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

Pore Size Monolith
Stationary Phase Protein A bonding phase
Temperature 2 to 45°C
Phase Affinity Separation
Matrix Monolithic silica
Length (Metric) 25 mm
Endcapped No
pH 1.5 to 7.5
Material PEEK
Technique HPLC, LC/MS
Surface Area 120 m2/g
Max. Pressure 200 bar
273

MilliporeSigma™ Chromolith™ High Resolution RP-18 Endcapped HPLC Column

Chromolith™ High Resolution 2 mm I.D. columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilan with full end-capping.

Pore Size Monolith
Type Column
Carbon Load 18%
Stationary Phase C18; Octadecylsilane (ODS) bonding phase
Temperature 50°C max.
Phase Reversed Phase
Matrix Highly porous monolithic silica
Endcapped Yes
Diameter (Metric) 2 mm
pH 2 to 7.5
Technique HPLC, MS
Max. Pressure 200 bar
274

MilliporeSigma™ Chromolith™ WP 300 RP-18 HPLC Column

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

Pore Size Monolith
Stationary Phase C18 bonding phase
Temperature 60°C max.
Phase Reversed Phase
Endcapped No
Material PEEK
Surface Area 300 m2/g
Max. Pressure 200 bar
Type Column
Carbon Load 18%
Matrix Monolithic silica
Diameter (Metric) 2 mm
pH 2 to 7.5
Technique HPLC, LC/MS, UHPLC, UHPLC-MS
USP Type L1
275

MilliporeSigma™ Chromolith™ WP 300 Epoxy HPLC Column, 2 mm

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

Pore Size Monolith
Type Column
Carbon Load 5 to 6%
Stationary Phase Epoxy bonding phase
Temperature 2 to 60°C
Matrix Monolithic silica
Endcapped No
Diameter (Metric) 2 mm
pH 1.5 to 7.5
Material PEEK
Technique HPLC, LC/MS, UHPLC, UHPLC-MS
Surface Area 120 m2/g
Max. Pressure 200 bar
276

Thermo Scientific™ Hypersil™ PREP BDS C8 HPLC Columns

Scale up to prep LC using Hypersil PREP C8 BDS HPLC columns which offers selectivity similar to C18 but with reduced retention in analytical and preparative scale.

Pore Size 145 Å
Carbon Load 7%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Base-Deactivated Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 185 m²/g
USP Type L7
277

Thermo Scientific™ Hypersil™ PREP BDS Phenyl HPLC Columns

Achieve unique selectivity for aromatic and slightly polar compounds with a base-deactivated Hypersil PREP Phenyl BDS prep LC column, suitable for USP L11 applications.

Pore Size 145 Å
Particle Size 5 μm
Carbon Load 5%
Stationary Phase Phenyl (Ph)
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Base-Deactivated Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 185 m²/g
USP Type L11
278

Thermo Scientific™ Hypersil™ PREP ODS C18 HPLC Columns

Perfect for routine workflows, our Hypersil PREP ODS preparative LC column is a general purpose C18 used in a broad range of USP L1 applications.

Pore Size 130 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 195 m²/g
USP Type L1
279

Thermo Scientific™ Hypersil™ PREP Si HPLC Columns

Separate nonpolar and moderately polar organic compounds with Hypersil PREP Si prep LC columns for normal phase methods, suitable for USP L3 applications.

Pore Size 130 Å
Particle Size 5 μm
Carbon Load 0%
Stationary Phase Silica
Temperature 60°C
Phase Normal Phase
Endcapped No
Packing Material Spherical, Fully Porous Silica
Column Type Normal Phase
pH 2 to 7
Surface Area 195 m²/g
USP Type L3
280

Thermo Scientific™ Hypersil™ PREP HS C8 HPLC Columns

Use these preparative columns for scale-up purification with high phase loading. They are suitable for all USP-L7 applications.

Pore Size 100 Å
Carbon Load 7%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 350 m²/g
USP Type L7
281

Thermo Scientific™ Hypersil™ PREP HS Phenyl HPLC Columns

Achieve unique selectivity for aromatic and slightly polar compounds with high surface area Hypersil PREP HS Phenyl prep LC columns for USP L11 applications.

Pore Size 100 Å
Particle Size 5 μm
Carbon Load 9%
Stationary Phase Phenyl (Ph)
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 350 m²/g
USP Type L11
282

Thermo Scientific™ Hypersil GOLD™ PREP C8 HPLC Columns

Get exceptional peak shape and resolution in HPLC and LC/MS using generic gradients. Use for analytes with medium hydrophobicity or when less hydrophobicity than C18 is needed.

Pore Size 175 Å
Particle Size 5 μm
Carbon Load 8%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 220 m²/g
USP Type L7
283

Thermo Scientific™ Hypersil GOLD™ PREP aQ HPLC Columns

Stable in 100% aqueous mobile phase, Hypersil GOLD aQ polar endcapped C18 preparative HPLC columns provide enhanced retention and resolution of polar analytes.

Pore Size 175 Å
Particle Size 5 μm
Carbon Load 12%
Temperature 60°C
Phase Reversed Phase
Endcapped Polar
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 220 m²/g
USP Type L1
284

Thermo Scientific™ Hypercarb™ PREP HPLC Columns

Conquer difficult separations in both normal phase and reversed phase applications with unique Hypercarb Porous Graphitic Carbon preparative HPLC columns.

Pore Size 250 Å
Carbon Load 100%
Stationary Phase Porous Graphitic Carbon
Temperature 105°C
Phase Normal and Reversed Phase
Packing Material Spherical, Porous Graphitic Carbon
Column Type Polar Retention
pH 0 to 14
Surface Area 120 m²/g
USP Type L109
285

Thermo Scientific™ Syncronis™ C18 HPLC Columns

Robust separations with highly pure, high surface area silica that is double endcapped Syncronis C18 preparative HPLC columns with inertness toward basic compounds.

Pore Size 100 Å
Particle Size 5 μm
Carbon Load 16%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 320 m²/g
USP Type L1