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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 23 results
1

MilliporeSigma™ Chromolith™ Prep HPLC Columns
GSA_VA

Patented, monolithic silica technology allows ultra-fast and robust separations using standard HPLC systems

Type Capillary Column
Length (Metric) 100 mm
Diameter (Metric) 25 mm
Quantity 1/Pk.
Porosity 0.8
Surface Area 350 m2/g
2

MilliporeSigma™ Chromolith™ DIOL5 HPLC Column

Made of monolithic silica with a characteristic bimodal pore structure. MilliporeSigma Chromolith™ DIOL5 HPLC Column functions in reverse phase (when in RP mode) and normal phase with DIOL5 stationary phase. Available in multiple sizes.

Stationary Phase DIOL5
Packing Material Monolithic silica
Column Type Chromolith™ HPLC Column
Mobile Phase Compatibility RP (reversed-phase, when in mode) & NP (normal-phase)
Diameter (Metric) 4.6 mm
3

MilliporeSigma™ Chromolith™ CN HPLC Column

Made of monolithic silica with a characteristic bimodal pore structure. MilliporeSigma Chromolith™ CN HPLC Column functions in reverse phase (when in RP mode) and normal phase with CN stationary phase. Available in multiple sizes.

Stationary Phase CN
Packing Material Monolithic silica
Column Type Chromolith™ HPLC Column
Mobile Phase Compatibility RP (reversed-phase, when in mode) & NP (normal-phase)
Diameter (Metric) 4.6 mm
4

MilliporeSigma™ Chromolith™ SemiPrep Monolithic HPLC Columns

Patented, monolithic silica technology allows ultra-fast and robust separations using standard HPLC systems

Pore Size 130 Å (Mesopore Size), 2 μm (Macropore Size)
Temperature 45°C
Length (Metric) 100 mm
Quantity 1/Pk.
Surface Area 300 m²/g
Max. Pressure 2175 psi (150 bar)
Column Format Preparative Column
Particle Size Monolith
Type Column
Carbon Load 18%
Packing Material Silica
Diameter (Metric) 10 mm
pH 2 to 7.5
Porosity >80%
USP Type L1
5

MilliporeSigma™ Chromolith™ WP 300 Protein A HPLC Column

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

Pore Size Monolith
Stationary Phase Protein A bonding phase
Temperature 2 to 45°C
Phase Affinity Separation
Matrix Monolithic silica
Length (Metric) 25 mm
Endcapped No
pH 1.5 to 7.5
Material PEEK
Technique HPLC, LC/MS
Surface Area 120 m2/g
Max. Pressure 200 bar
6

MilliporeSigma™ Chromolith™ FastGradient RP-18 Endcapped HPLC Columns

Patented, monolithic silica technology allows ultra-fast and robust separations using standard HPLC systems

Stationary Phase C18
Phase Reversed Phase
Length (Metric) 50 mm
Endcapped Yes
Column Type Reversed Phase
Quantity 1/Pk.
Surface Area 300 m²/g
Column Format Column
Particle Size Monolith
Type Column
Carbon Load 18%
Packing Material Silica
Porosity >80%
USP Type L1
7

MilliporeSigma™ Chromolith™ Flash RP-18 Endcapped HPLC Columns
GSA_VA

Patented, monolithic silica technology allows ultra-fast and robust separations using standard HPLC systems

Stationary Phase C18
Phase Reversed Phase
Length (Metric) 25 mm
Endcapped Yes
Column Type Reversed Phase
Quantity 1/Pk.
Surface Area 300 m²/g
Column Format Column
Particle Size Monolith
Type Column
Carbon Load 18%
Packing Material Silica
Porosity >80%
USP Type L1
8

MilliporeSigma™ Chromolith™ WP 300 RP-18 HPLC Column

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

Pore Size Monolith
Stationary Phase C18 bonding phase
Temperature 60°C max.
Phase Reversed Phase
Endcapped No
Material PEEK
Surface Area 300 m2/g
Max. Pressure 200 bar
Type Column
Carbon Load 18%
Matrix Monolithic silica
Diameter (Metric) 2 mm
pH 2 to 7.5
Technique HPLC, LC/MS, UHPLC, UHPLC-MS
USP Type L1
9

MilliporeSigma™ Chromolith™ Performance RP-18 Endcapped HPLC Columns
GSA_VA

Patented, monolithic silica technology allows ultra-fast and robust separations using standard HPLC systems

Particle Size Monolith
Type Column
Carbon Load 18%
Stationary Phase C18
Phase Reversed Phase
Length (Metric) 100 mm
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
Quantity 1/Pk.
Surface Area 300 m²/g
USP Type L1
10

MilliporeSigma™ Chromolith™ HighResolution RP-18 Endcapped HPLC Columns

Patented, monolithic silica technology allows ultra-fast and robust separations using standard HPLC systems

Pore Size 150 Å (Mesopore Size), 1.15 μm (Macropore Size)
Stationary Phase RP 18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Quantity 1/Pk.
Surface Area 250 m²/g
Column Format Column
Particle Size Monolith
Type Column
Carbon Load 18%
Packing Material Monolith
Diameter (Metric) 4.6 mm
USP Type L1
11

MilliporeSigma™ Chromolith™ CapRod™ RP Monolith Capillary Columns

Patented, monolithic silica technology allows ultra-fast and robust separations using standard HPLC systems

Type Capillary Column
Quantity 1/Pk.
Surface Area 300 m2/g
12

MilliporeSigma™ Chromolith™ WP 300 Epoxy HPLC Column, 2 mm

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

Pore Size Monolith
Type Column
Carbon Load 5 to 6%
Stationary Phase Epoxy bonding phase
Temperature 2 to 60°C
Matrix Monolithic silica
Endcapped No
Diameter (Metric) 2 mm
pH 1.5 to 7.5
Material PEEK
Technique HPLC, LC/MS, UHPLC, UHPLC-MS
Surface Area 120 m2/g
Max. Pressure 200 bar
13

MilliporeSigma™ Chromolith™ High Resolution RP-18 Endcapped HPLC Column

Chromolith™ High Resolution 2 mm I.D. columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilan with full end-capping.

Pore Size Monolith
Type Column
Carbon Load 18%
Stationary Phase C18; Octadecylsilane (ODS) bonding phase
Temperature 50°C max.
Phase Reversed Phase
Matrix Highly porous monolithic silica
Endcapped Yes
Diameter (Metric) 2 mm
pH 2 to 7.5
Technique HPLC, MS
Max. Pressure 200 bar
14

MilliporeSigma™ Chromolith™ WP 300 Epoxy HPLC Guard Cartridges

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

15

MilliporeSigma™ Chromolith™ WP 300 RP-18 HPLC Guard Cartridges

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.