ION Biosciences' ION Essentials Ratiometric Calcium Flex kit combines Fura-2 AM with the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give you the flexibility needed to customize your assay. Ratiometry is not only optimal for imaging applications where quantification of intracellular Ca²⁺ concentrations is desired, it also reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.