Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Human Orexin A ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10% |
| Gene Symbol | HCRT |
| Total Assay Time | 2 hr 30 min |
| Assay Sensitivity | 37.5 pg/mL |
| Storage Requirements | Refer to product documentation for component specific storage temperature. |
| Assay Range | 62.5-4000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | O43612 |
| Regulatory Status | RUO |
| Target | Orexin A |
| Kit Contents | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer |
| Gene Alias | HCRT; Hcrt1; Hcrt2; Hypocretin; Hypocretin (orexin) neuropeptide; hypocretin neuropeptide precursor; hypocretin-1; Hypocretin-2; NRCLP1; orexin; orexin A; orexin B; orexin-A; Orexin-B; ORX; OX; PPORX; PPOX; prepro-orexinHCRT; Hcrt1; Hcrt2; Hypocretin; Hypocretin (orexin) neuropeptide; hypocretin neuropeptide precursor; hypocretin-1; Hypocretin-2; NRCLP1; orexin; orexin A; orexin B; orexin-A; Orexin-B; ORX; OX; PPORX; PPOX; prepro-orexin; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 3060 |
| Sample Type | Plasma,Serum |
| Label or Dye | Biotin |
Invitrogen™ Human TNF alpha Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Gene Symbol | Tnf |
| Total Assay Time | 24 hr 30 min |
| Sample Volume | Plasma 100 μL, Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity | 4 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 4-500 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 45 min |
| Accession Number | P01375 |
| Regulatory Status | RUO |
| Target | TNF alpha |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X), Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 7124 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | Tumor Necrosis Factor alpha, tnf-a, tnf-alpha |
Invitrogen™ Human IL-10 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Protein Aliases | IL-10, Cytokine synthesis inhibitory factor, CSIF |
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human |
| Conjugate | HRP |
| Interassay CV | 2.8% |
| Intraassay CV | 3.3% |
| Gene Symbol | IL10 |
| Total Assay Time | 4 hr 30 min |
| Assay Sensitivity | 1 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 11-1335 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P22301 |
| Regulatory Status | RUO |
| Target | IL-10 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample diluent, Incubation Buffer, HRP conjugated Detection Antibody, Conjugate Diluent, Wash Buffer, Chromogen, Stop Solution, Controls,Standard,Sample diluent,Incubation Buffer,HRP conjugated Detection Antibody,Conjugate Diluent,Wash Buffer,Chromogen,Stop Solution,Controls |
| Gene Alias | CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; CSIF; GVHDS; IL-10A; TGIF; IL10 |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 3586 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Rat Prolactin ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | PRL |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.41 ng/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.41-100 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P01237 |
| Regulatory Status | RUO |
| Target | Prolactin |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 24683 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Human IkB-alpha ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | NFKBIA |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.65 ng/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.65-150 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P25963 |
| Regulatory Status | RUO |
| Target | IkB alpha |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4792 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Total Cholesterol (TC) Colorimetric Assay Kit (single reagent, COD-PAP method)
These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Human Granzyme B ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | 6.8% |
| Intraassay CV | 6.2% |
| Gene Symbol | GZMB |
| Total Assay Time | 2 hr 30 min |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <0.43 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 0.7-480 pg/mL |
| Test Time | 1 hr |
| Accession Number | P10144 |
| Regulatory Status | RUO |
| Target | Granzyme B |
| Kit Contents | Pre-coated 96 well plate, Standard, Detection antibody (monoclonal antibody to human Granzyme B), Biotin-Conjugate anti-human Granzyme B monoclonal antibody, Streptavidin-HRP, human Granzyme B Standard lyophilized, Wash Buffer Concentrate 20x, Dilution Buffer Concentrate 5x, Substrate Solution, Stop Solution, Adhesive Films,Standard,Detection antibody (monoclonal antibody to human Granzyme B),Biotin-Conjugate anti-human Granzyme B monoclonal antibody,Streptavidin-HRP,human Granzyme B Standard lyophilized,Wash Buffer Concentrate 20x,Dilution Buffer Concentrate 5x,Substrate Solution,Stop Solution,Adhesive Films |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3002 |
| Sample Type | Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Human STAT5 alpha/beta InstantOne™ ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Immunoassay Kit Format | Coated ELISA Kit |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Gene Symbol | STAT5A, STAT5B |
| Total Assay Time | 1 hr 10 min |
| Storage Requirements | 2-8°C |
| Test Time | 1 hr 20 min |
| Accession Number | P42229, P51692 |
| Regulatory Status | RUO |
| Target | STAT5 alpha/beta |
| Kit Contents | Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal,Detection Antibody Reagent,InstantOne™ Assay Plate,Cell Lysis Buffer,Enhancer Solution,Wash Buffer (10X),Substrate Reagent (TMB),Stop Solution,Positive Control Cell Lysate (lyophilized),Plate seal |
| Gene Alias | AA959963; Mammary gland factor; mammary gland factor STAT5A; mammary gland factor STAT5B; mammary gland factor; transcription factor; MGF; Mpf; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5a; Signal transducer and activator of transcription 5B; STA5B; STAT; STAT5; Stat5a; Stat5b; transcription factor STAT5BAA959963; Mammary gland factor; mammary gland factor STAT5A; mammary gland factor STAT5B; mammary gland factor; transcription factor; MGF; Mpf; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5a; Signal transducer and activator of transcription 5B; STA5B; STAT; STAT5; Stat5a; Stat5b; transcription factor STAT5B; STAT5; MGF |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 6776, 6777 |
| Sample Type | Cell Lysates |
| Label or Dye | Biotin |
Invitrogen™ Human IL-6 Receptor (Soluble) ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 2.2% |
| Intraassay CV | 1.7% |
| Gene Symbol | IL6R |
| Total Assay Time | 2 hr 10 min |
| Sample Volume | Amniotic Fluid 20 μL, Plasma 10 μL, Serum 10 μL, Supernatant 20 μL, Urine 20 μL |
| Assay Sensitivity | 0.01 ng/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 0.08-5.0 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P08887 |
| Regulatory Status | RUO |
| Target | IL-6 Receptor (CD126) |
| Kit Contents | Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3570 |
| Sample Type | Amniotic Fluid,Plasma,Serum,Supernatant,Urine |
| Label or Dye | HRP |
| Synonym | CD126, Membrane glycoprotein 80 |
R&D Systems™ Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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For the quantitative determination of mouse C-Reactive Protein (CRP) concentrations in cell culture supernates, tissue lysates, serum, and plasma
Invitrogen™ Human PTX-3 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | PTX3 |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.08 ng/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.08-20 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P26022 |
| Regulatory Status | RUO |
| Target | PTX3 |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 5806 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Human Cu/ZnSOD ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 5.8% |
| Intraassay CV | 5.1% |
| Gene Symbol | SOD1 |
| Total Assay Time | 1 hr 10 min |
| Sample Volume | Amniotic Fluid 10 μL, Fetal Umbilical Vein Blood 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Urine 10 μL |
| Assay Sensitivity | 0.04 ng/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 0.08-5.0 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P00441 |
| Regulatory Status | RUO |
| Target | SOD1 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 6647 |
| Sample Type | Amniotic Fluid,Fetal Umbilical Vein Blood,Plasma,Serum,Supernatant,Urine |
| Label or Dye | HRP |
| Synonym | Cu/Zn Superoxide Dismutase |
Invitrogen™ Human CD63 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | CD63 |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 14 pg/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 14-3000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P08962 |
| Regulatory Status | RUO |
| Target | CD63 |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 967 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
R&D Systems™ Human FABP2/I-FABP Quantikine ELISA Kit
Human FABP2/I-FABP Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Invitrogen™ Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Chemical |
|---|---|
| Conjugate | HRP |
| Description | Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA Kit |
| Interassay CV | 8.4% |
| Intraassay CV | 7% |
| Total Assay Time | 2 hr 30 min |
| Sample Volume | Fecal Extract 50 μL, Plasma 1 μL, Saliva 25 μL, Serum 1 μL, Supernatant 50 μL, Urine 25 μL |
| Assay Sensitivity | 90.9 pg/mL |
| Storage Requirements | -20°C |
| Research Discipline | Reproductive Biology |
| Assay Range | 96-60000 pg/mL |
| For Use With (Application) | ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti |
| Test Time | 1 hr 20 min |
| Detection Method | Absorbance at 450 nm |
| Regulatory Status | RUO |
| Target | DHEA-S |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Sample Type | Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine |
| Label or Dye | HRP |