Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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BD Human IL-5 ELISA Set
To develop enzyme-linked immunosorbent assays (ELISA) for natural or recombinant human IL-5 in serum, plasma, and cell culture supernatants
Invitrogen™ Rat Angiopoietin-1 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Angpt1 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.4 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.4 to 100 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 1110046O21Rik; AGP1; AGPT; Agpt1; Ang1; Ang-1; angiopoietin 1; angiopoietin-1; ANGPT1; endothelial growth factor; KIAA0003 |
| Regulatory Status | RUO |
| Target | Angiopoietin 1 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 89807 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Rat TNF-alpha ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Tnf |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 25 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 25 to 20,000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1 F; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor |
| Regulatory Status | RUO |
| Target | TNF alpha |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 24835 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human IL-10 ProQuantum Immunoassay Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | FAM™ |
|---|---|
| Gene Symbol | IL10 |
| Total Assay Time | 2 hrs |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.05 pg/mL |
| Storage Requirements | -20°C |
| Assay Range | 0.064 to 5,000 pg/mL |
| Test Time | 30 mins |
| Accession Number | P22301 |
| Regulatory Status | RUO |
| Target | IL-10 |
| Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase,Fetal Bovine Serum |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 3586 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
Invitrogen™ Human MCP-1 ProQuantum Immunoassay Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM™ |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hrs |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.05 pg/mL |
| Storage Requirements | Frozen at -20°C |
| Assay Range | 0.064 to 5000 |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 mins |
| Detection Method | Taqman™ Real-time PCR |
| Regulatory Status | RUO |
| Target | CCL2 (MCP-1) |
| Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 6347 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
Invitrogen™ Human MCP-2 ProQuantum Immunoassay Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM™ |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hrs |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.01 pg/mL |
| Storage Requirements | Frozen at -20°C |
| Assay Range | 0.0128 to 5000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 mins |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P80075 |
| Regulatory Status | RUO |
| Target | CCL8 (MCP2) |
| Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 6355 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
Novus Biologicals™ Rat Adrenomedullin/ADM ELISA Kit (Colorimetric)
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Invitrogen™ Human Angiopoietin 1 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Family | Kinase & Inhibitors |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Intraassay CV | <10 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 33 μL, Serum 33 μL, Supernatant 100 μL |
| Assay Sensitivity | 30 pg/mL |
| Research Discipline | Angiogenesis |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Assay | Solid-phase sandwich ELISA |
| Target | Angiopoietin 1 |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Label or Dye | HRP |
| Interassay CV | <12% |
| Gene Symbol | AGP1,AGPT,ANG1 |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 74.07 to 18,000 pg/mL |
| Accession Number | Q15389 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 284 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | AGP1, AGPT, ANG1 |
Invitrogen™ Human Ficolin-3 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | FCN3 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.25 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.25 to 50 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; FCN3; FCNH; ficolin 3; Ficolin-3; HAKA1; Hakata antigen; MGC22543 |
| Regulatory Status | RUO |
| Target | Ficolin 3 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 8547 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Novus Biologicals™ VCAM-1/CD106 Antibody Pair [HRP]
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Mouse Monoclonal Antibody Pair
| Host Species | Mouse |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Gene Symbol | VCAM1 |
| Assay Sensitivity | 46.88-3000 pg/mL |
| Storage Requirements | Storage is content dependent. |
| Research Discipline | Cancer, Mesenchymal Stem Cell Markers, Phospho Specific, Stem Cell Markers |
| Assay Range | 46.88 pg/mL |
| For Use With (Application) | Sandwich ELISA |
| Assay | Sandwich ELISA |
| Regulatory Status | RUO |
| Target | VCAM-1/CD106 |
| Specificity | This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human VCAM-1/CD106. |
| Gene Alias | CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1 |
| Product Type | Antibody Pairs |
| Gene ID (Entrez) | 7412 |
Invitrogen™ Human DKK3 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Bovine IL-4 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Novus Biologicals™ pIgR Antibody Pair [HRP]
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Mouse Monoclonal Antibody Pair
| Host Species | Mouse |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Gene Symbol | PIGR |
| Assay Sensitivity | 62.5-4000 pg/mL |
| Storage Requirements | Storage is content dependent. |
| Research Discipline | Immunology |
| Assay Range | 62.5 pg/mL |
| For Use With (Application) | Sandwich ELISA |
| Assay | Sandwich ELISA |
| Regulatory Status | RUO |
| Target | pIgR |
| Specificity | This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human pIgR. |
| Kit Contents | |
| Gene Alias | FLJ22667, hepatocellular carcinoma associated protein TB6, Hepatocellular carcinoma-associated protein TB6, MGC125361, MGC125362, PIgR, Poly-Ig receptor, polymeric immunoglobulin receptor |
| Product Type | Antibody Pairs |
| Gene ID (Entrez) | 5284 |
| Technique | 1. Capture Antibody: Dilute to a working concentration of 2.0 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 1.00 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute standard powder with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended. |
Novus Biologicals™ IL-7 Antibody Pair [HRP]
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Polyclonal/Monoclonal Antibody Pair
| Host Species | Rabbit/Mouse |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Gene Symbol | IL7 |
| Assay Sensitivity | 3.91-250 pg/mL |
| Storage Requirements | Storage is content dependent. |
| Research Discipline | Biologically Active Proteins, Cell Cycle and Replication, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Stem Cell Markers |
| Assay Range | 3.91 pg/mL |
| For Use With (Application) | Sandwich ELISA |
| Assay | Sandwich ELISA |
| Regulatory Status | RUO |
| Target | IL-7 |
| Specificity | This solid phase sandwich ELISA utilizes an antibody specific for Human IL-7. |
| Gene Alias | IL-7interleukin-7, interleukin 7 |
| Product Type | Antibody Pairs |
| Gene ID (Entrez) | 3574 |
Novus Biologicals™ CD30/TNFRSF8 Antibody Pair [HRP]
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Monoclonal Antibody Pair
| Host Species | Rabbit/Mouse |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Gene Symbol | TNFRSF8 |
| Assay Sensitivity | 23.44-1500 pg/mL |
| Storage Requirements | Storage is content dependent. |
| Research Discipline | Apoptosis, B Cell Development and Differentiation Markers, Cell Cycle and Replication, Immunology, Stem Cell Markers |
| Assay Range | 24 pg/mL |
| For Use With (Application) | Sandwich ELISA |
| Assay | Sandwich ELISA |
| Regulatory Status | RUO |
| Target | CD30/TNFRSF8 |
| Specificity | This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD30/TNFRSF8. |
| Kit Contents | |
| Gene Alias | CD30, CD30 antigen, CD30KI-1, CD30L receptor, cytokine receptor CD30, D1S166EKi-1, Ki-1 antigen, Lymphocyte activation antigen CD30, tumor necrosis factor receptor superfamily member 8, tumor necrosis factor receptor superfamily, member 8 |
| Product Type | Antibody Pairs |
| Gene ID (Entrez) | 943 |