Protein Method Blocking Buffers
Vector Laboratories Mouse On Mouse (M.O.M.) Blocking Reagent 1 ml
M.O.M. Mouse Ig Blocking Reagent is used to block endogenous mouse antibody in tissue sections. A component of the M.O.M. Kits, this reagent provide optimal staining results when it is paired with the M.O.M. Biotinylated Anti-Mouse Ig Reagent or the biotin-free, one-step micropolymer detection reagent, ImmPRESS Anti-Mouse Ig Reagent (MPX-2402). This reagent is supplied as a 1 ml concentrate, sufficient to prepare about 25 ml of working solution, or enough to stain 125-250 sections.
Teknova 10mM Tris-HCl with 0.1% Tween-20, pH 8.5. 1mL/Tube, 50 Pack, Sterile.
10mM Tris-HCl, 0.1% Tween-20
LI COR BIOTECH LLC Intercept (TBS) Blocking Buffer and Diluent Kit
Try Intercept (TBS) Blocking Buffer and Intercept T20 (TBS) Antibody Diluent for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability. Intercept T20 Antibody Diluents improve the specificity of the primary and secondary antibodies, reducing off-target effects. They are preformulated with Tween 20. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation. This kit includes Intercept (TBS) Blocking Buffer (1 x 500 mL) and Intercept T20 (TBS) Antibody Diluent (2 x 500 mL)
Surmodics Inc Phosphate Buffered Saline/Casein Block & Diluent-5X, 1L
BioFX™ Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
Rockland Immunochemicals BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.
Biotium TrueBlack Plus Lipofuscin Autofluorescence Quencher | 500uL
TrueBlack Plus Lipofuscin Autofluorescence Quencher | 40x concentrated solution in DMSO | 500uL
Sigma Aldrich Fine Chemicals Biosciences Carbonate-Bicarbonate Buffer capsule | 50CAP
Carbonate-Bicarbonate Buffer capsule | 50CAP
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 1.0G
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Abcam Protein Block, 125ML
Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required
The product is subject to the following: Abcam Restricted Use Statement
LI COR BIOTECH LLC Intercept (PBS) Blocking Buffer (3 x 500 mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Santa Cruz Biotechnology BLOTTO NON-FAT DRY MILK
Blotto, Non-fat dry milk; Lyophilized powder; Contains Santa Cruz Biotechnology immunoanalytical grade blotto; Using w/phospho-specific antibodies: add 50mM NaF to Blotto to inhibit phosphatase activity