Protein Method Blocking Buffers
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml) - MB-070-003
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Teknova 0.01M PBS with 0.02% Tween-80. 1000mL, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Bioworld Casein-Blocking Buffer in PBS w/ Azide, 500 mL
Product DescriptionCasein-blocking buffer is 3% in PBS with 0.02% sodium azide. It is a liquid stored between temperatures of -30°C to -10°C, and is used for Western blot and ELISA. Buffer used for blocking excess binding sites on membranes and microplates in antibody-based detection methods.
Bioworld Casein-Blocking Buffer in TBS, 1 L
Casein Blocking Buffer in TBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane. Technical Information: 1% Casein in TBS
Genetex Inc GeneTex Blocking Buffer, 5% Milk For WB and ELISA, Reduces Background Interference, 250 mL
GeneTex Blocking Buffer, TBS with 5% Milk is a ready-to-use solution that enhances assay sensitivity by minimizing non-specific background interference in immunological detection methods. It is specifically validated for Western blotting (WB) and ELISA, improving signal-to-noise ratios by binding to sites of nonspecific interaction. This liquid formulation comes in a 250 mL volume, making it ideal for various applications including immunohistochemistry.
LAB BIOREAGENTS Strongblock I 500ML
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
SCANTIBODIES LABORATORY INC HETEROPHILIC BLOCKING TUBE (3IX762) 50PK
The 3IX762 HBT (Heterophilic Blocking Tube) from Scantibodies Laboratory, Inc. contains a unique blocking reagent composed of specific binders which inactivate heterophilic antibodies. Once the specific binders have bound to the heterophilic antibodies, the antibodies are no longer able to cause immunoassay interference. The reagent is in the form of a lyophilized pellet at the bottom of the tube and each tube contains enough reagents to inactivate the heterophilic antibodies in 500 μl of sample material. The HBT also allows for the rapid and simple elimination of false positive heterophilic interference in plasma or serum for immunoassays (i.e., FSH, LH, Prolactin, TSH, Ferritin, CEA, AFP, hCG, HBsAg, CK-MB, CA 125, CA 19-9, NSE, etc.).
Alkali Scientific Dry Powder Milk, 10 Kg
Dry Powder Milk, 10 Kg, is a non-fat milk powder designed for use in microbiological and biochemical laboratories for preparing culture media and fermentation processes. This larger bulk size is ideal for high-volume research facilities or production environments that require consistent nutrient-rich media for microbial growth. The powder is an essential ingredient in creating media for the growth of bacteria, fungi, and other microorganisms. It provides the necessary nutrients for metabolic processes and supports the growth of cells in culture. The 10 kg package offers a cost-effective solution for laboratories that require significant quantities of milk powder for routine media preparation and microbial studies.
Medchemexpress LLC (+)-KCC2 blocker 1 | 1228439-71-1 | 98.7% | 415.50 | 1 MG
(+)-KCC2 blocker 1 is a selective K+-Cl- cotransporter KCC2 blocker with an IC50 of 0.4 μM. It is a benzyl prolinate and an enantiomer of KCC2 blocker 1. It is intended for research use only and is not sold to patients.
- Selective K+-Cl- cotransporter KCC2 blocker
- IC50 of 0.4 μM for KCC2
- Benzyl prolinate
- Enantiomer of KCC2 blocker 1
Biotang Inc BLOTTO Antifoam in TBS (with azide), 250 ml
BLOTTO Antifoam in TBS (with azide), 250 ml.
Biotang Inc GELATIN-Blocking Buffer in TBS1% Gelatin, 500 ml
GELATIN-Blocking Buffer in TBS1% Gelatin, 500 ml.
Medchemexpress LLC KCC2 blocker 1 | 1228439-36-8 | 98.2% | 415.50 | 5 MG
KCC2 blocker 1 is an orally active and selective K+-Cl- cotransporter KCC2 blocker with an IC50 of 1 μM. It is a benzyl prolinate.
- Orally active and selective K+-Cl- cotransporter KCC2 blocker
- IC50 of 1 μM (KCC2)
- Benzyl prolinate
- It has a t1/2 of 0.3 hours, a CL of 26 mL/min/kg, a C max of 457 ng/mL and a AUC of 726 ng·h/mL for male Wistar rats.
Alkali Scientific Blocking Solution Starter Kit, Contains 100mL of Each Solution: Blotto Tween 20 Blocking Solution in TBS (with Azide), BSA Tween 20 Blocking Solution in TBS, Casein Blocking Solution in TBS
The Blocking Solution Starter Kit includes 100mL of each of three commonly used blocking solutions: Blotto Tween 20 Blocking Solution in TBS (with Azide), BSA Tween 20 Blocking Solution in TBS, and Casein Blocking Solution in TBS. Each solution is pre-formulated and ready to use, providing flexibility in choosing the most appropriate blocker for different immunoassays. The kit is ideal for labs that perform various assays requiring protein blocking to reduce background noise and enhance signal clarity. With multiple formulations, this kit provides a comprehensive solution for protein blocking in applications such as ELISA, Western Blot, and immunohistochemistry.
Seracare Life Sciences Inc 10X TBS W/ 0 5% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 0.5% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures.