Fisher Scientific
Products
Gel Electrophoresis Equipment and Supplies
Gel Electrophoresis Reagents and Buffers
Gel Electrophoresis Buffers and Diluents

Gel Electrophoresis Buffers and Diluents

Buffer and diluent solutions to prepare, dilute, and deliver protein and nucleic acid samples to electrophoresis gels for separation and analysis; includes solutions to stabilize, control the concentration of, denature, and reduce sample proteins.

Quantity

1 Kit (2)
1 L (44)
1 g (2)
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1 mL (2)
1 x 28 mL + 100 Wells (1)
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10 L (1)
10 Packets (1)
10 g (1)
10 mL (1)
10 mg (1)
10 x 1 L (1)
10 x 96 Tests (2)
100 g (2)
100 mL (32)
100g (1)
12 Vials (1)
125 mL (2)
15 x 6 mL (1)
1kg (1)
2 L (1)
20 L (2)
20 mL (1)
200 mL (1)
21 mL (1)
230.8 mL (1)
24 mL (1)
25 g (2)
25 mL (6)
250 mL (33)
25g (1)
3 Pack (3)
3 bottles, 25 mL (1)
3 mL (1)
3.8 L (1)
30 mL (2)
3800 mL (1)
4 L (10)
4 Pack (1)
4 mL (3)
4 x 27 mL (1)
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4 x 60 mL (1)
40 mL (1)
400 mL (1)
5 lb. (1)
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5 x 100 mL (1)
5 x 21 mL (1)
5/Pk. (1)
50 Cs. (5)
50 mL (6)
500 g (1)
500 mL (72)
6 Pack (1)
60 mL (1)
7 mL (1)
90 mL (1)
90 mL (3 oz.) (1)
961.5 mL (1)
Each (1)

Physical Form

Crystalline (5)
Liquid (175)
Lyophilized (1)
Powder (3)
Solution (17)
Viscous Liquid (1)

Product Type

Acetate Buffer (2)
Acrylamide (1)
Antioxidant (1)
Buffer (1)
Carrier Ampholyte (5)
Coating Buffer (1)
DIGE Gel and Buffer Kit (2)
DNA Gel Loading Dye (1)
DNA Loading Dye (1)
DNA Recovery Buffer (1)
DNA Sample Loading Buffer (5)
DNA Stain (1)
Digitonin Solution (1)
Electrophoresis Clean-up Kit (1)
ExcelGel SDS Buffer strips (1)
Fluorescent Compatible Sample Buffer (1)
Gel Loading Buffer (1)
IEF Anode Buffer (1)
IEF Buffer Kit (1)
IEF Cathode Buffer (2)
IEF Sample Buffer (2)
IPG Buffer (5)
LDS Sample Buffer (5)
Lane Marker Non-Reducing Sample Buffer (1)
Lane Marker Reducing Sample Buffer (1)
Loading Buffer (7)
PAGE Cathode Buffer Additive (1)
PAGE G-250 Sample Additive (1)
PAGE Running Buffer (1)
PAGE Running Buffer Kit (1)
PAGE Sample Buffer (1)
PAGE Sample Prep Kit (1)
Pharmalyte Carrier Ampholyte (5)
Polyacrylamide Gel Strengthener Concentrate (1)
Protein Sample Loading Buffer (4)
RNA Sample Loading Buffer (1)
RNA Sample Loading Buffer-Formaldehyde (1)
Reaction Buffer (1)
Reagent Kit of Resolving Solution (1)
SDS PAGE Clean-up Kit (1)
SDS Running Buffer (5)
SDS Solution (2)
SDS-PAGE Sample Prep Kit (1)
SSC (2)
Sample Loading Buffer (9)
Sample Reducing Agent (4)
Sodium Dodecyl Sulfate (1)
Transfer Buffer (2)
Triple-Dye Loading Buffer (1)
Western Blot Transfer Buffer (4)
Zymogram Developing Buffer (1)
Zymogram Renaturing Buffer (1)

Concentration

0.1 M (5)
0.1M buffer soln. (3)
0.2M buffer soln. (35)
0.5M buffer soln. (33)
10 mM (1)
100 mM (1)
10X (3)
1M buffer soln. (2)
1X (2)
200mM buffer soln. (2)
20X (3)
4X (3)
50X (1)
5M (1)
Functional (1)

Molecular Weight (g/mol)

119.976 (25)
136.084 (6)
163.217 (2)
174.17 (1)
199.187 (2)
204.222 (2)
214.024 (3)
221.315 (1)
257.301 (2)
265.256 (1)
285.252 (1)
294.10 (18)
330.414 (1)
35.046 (1)
367.45 g/mol (1)
39.997 (1)
68.079 (5)
75.067 (9)
82.034 (2)
92.094 (2)

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Quantity

1 Kit (2)
1 L (44)
1 g (2)
1 kg (1)
1 mL (2)
1 x 28 mL + 100 Wells (1)
1.9 L (1)
10 L (1)
10 Packets (1)
10 g (1)
10 mL (1)
10 mg (1)
10 x 1 L (1)
10 x 96 Tests (2)
100 g (2)
100 mL (32)
100g (1)
12 Vials (1)
125 mL (2)
15 x 6 mL (1)
1kg (1)
2 L (1)
20 L (2)
20 mL (1)
200 mL (1)
21 mL (1)
230.8 mL (1)
24 mL (1)
25 g (2)
25 mL (6)
250 mL (33)
25g (1)
3 Pack (3)
3 bottles, 25 mL (1)
3 mL (1)
3.8 L (1)
30 mL (2)
3800 mL (1)
4 L (10)
4 Pack (1)
4 mL (3)
4 x 27 mL (1)
4 x 4 L (3)
4 x 60 mL (1)
40 mL (1)
400 mL (1)
5 lb. (1)
5 x 10 mL (1)
5 x 100 mL (1)
5 x 21 mL (1)
5/Pk. (1)
50 Cs. (5)
50 mL (6)
500 g (1)
500 mL (72)
6 Pack (1)
60 mL (1)
7 mL (1)
90 mL (1)
90 mL (3 oz.) (1)
961.5 mL (1)
Each (1)

Physical Form

Crystalline (5)
Liquid (175)
Lyophilized (1)
Powder (3)
Solution (17)
Viscous Liquid (1)

Product Type

Acetate Buffer (2)
Acrylamide (1)
Antioxidant (1)
Buffer (1)
Carrier Ampholyte (5)
Coating Buffer (1)
DIGE Gel and Buffer Kit (2)
DNA Gel Loading Dye (1)
DNA Loading Dye (1)
DNA Recovery Buffer (1)
DNA Sample Loading Buffer (5)
DNA Stain (1)
Digitonin Solution (1)
Electrophoresis Clean-up Kit (1)
ExcelGel SDS Buffer strips (1)
Fluorescent Compatible Sample Buffer (1)
Gel Loading Buffer (1)
IEF Anode Buffer (1)
IEF Buffer Kit (1)
IEF Cathode Buffer (2)
IEF Sample Buffer (2)
IPG Buffer (5)
LDS Sample Buffer (5)
Lane Marker Non-Reducing Sample Buffer (1)
Lane Marker Reducing Sample Buffer (1)
Loading Buffer (7)
PAGE Cathode Buffer Additive (1)
PAGE G-250 Sample Additive (1)
PAGE Running Buffer (1)
PAGE Running Buffer Kit (1)
PAGE Sample Buffer (1)
PAGE Sample Prep Kit (1)
Pharmalyte Carrier Ampholyte (5)
Polyacrylamide Gel Strengthener Concentrate (1)
Protein Sample Loading Buffer (4)
RNA Sample Loading Buffer (1)
RNA Sample Loading Buffer-Formaldehyde (1)
Reaction Buffer (1)
Reagent Kit of Resolving Solution (1)
SDS PAGE Clean-up Kit (1)
SDS Running Buffer (5)
SDS Solution (2)
SDS-PAGE Sample Prep Kit (1)
SSC (2)
Sample Loading Buffer (9)
Sample Reducing Agent (4)
Sodium Dodecyl Sulfate (1)
Transfer Buffer (2)
Triple-Dye Loading Buffer (1)
Western Blot Transfer Buffer (4)
Zymogram Developing Buffer (1)
Zymogram Renaturing Buffer (1)

Concentration

0.1 M (5)
0.1M buffer soln. (3)
0.2M buffer soln. (35)
0.5M buffer soln. (33)
10 mM (1)
100 mM (1)
10X (3)
1M buffer soln. (2)
1X (2)
200mM buffer soln. (2)
20X (3)
4X (3)
50X (1)
5M (1)
Functional (1)

Molecular Weight (g/mol)

119.976 (25)
136.084 (6)
163.217 (2)
174.17 (1)
199.187 (2)
204.222 (2)
214.024 (3)
221.315 (1)
257.301 (2)
265.256 (1)
285.252 (1)
294.10 (18)
330.414 (1)
35.046 (1)
367.45 g/mol (1)
39.997 (1)
68.079 (5)
75.067 (9)
82.034 (2)
92.094 (2)

pH

10 (1)
2.5 (4)
2.87 (1)
2.9 to 3 (2)
3 (1)
3.0 (4)
3.5 (6)
3.5 to 3.6 (1)
4.0 (2)
4.0 (0.05M H₂O soln. 25°C) (2)
4.5 (2)
5 (1)
5.0 (4)
5.2 (2)
5.5 (2)
5.9 to 6.3 (1)
6 (3)
6.0 (3)
6.5 (2)
6.8 (2)
7 (7)
7.0 (6)
7.2 (1)
7.2 ± 0.2 (1)
7.4 (2)
7.5 (8)
8 (1)
8.0 (2)
8.2 (1)
8.4 to 8.6 (at 25°C) (2)
8.5 (1)
9.0 (1)
9.5 (2)

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For Use With (Application)

Mycoplasma detection (1)
APHA for Chlorine and Chlorine Dioxide (Amperometric Method) (3)
Compendial Testing (1)
Designed for testing in the food, dairy, pharmaceutical, water, and cosmetic industries (5)
ELISA (7)
For AOAC Method 946.01 (2)
For AOAC Method 968.13 (2)
For disinfectant preparation and dilution (1)
For frozen storage of competent cells (2)
For the quantitative measurement of the percentage of total Glycated Hemoglobins in whole Blood, for in-vitro diagnostic use (1)
For washing microplates (1)
General Laboratory Reagent (1)
Intracellular staining followed by flow cytometric analysis (1)
Pharma & Biopharma; Protein Assays and Analysis; Cellular Imaging (2)
Purification of HisTag fusion proteins (1)
Sputum Testing (2)
The Antigen Retrieval Protocol is Recommended for Use in Tissues That Have Been Fixed in Formalin Only. Ensure That The Fixed Sections Are Adequately Embedded in Paraffin. Cut Tissue Sections to 4-5 Microns. Preparation of Working Solutions 1. The 10 x Concentrated Format Should be Diluted Tenfold with Distilled or Deionized Water. 2. Mix One Part of Concentrated Antigen Retrieval Solution with Nine Parts of Deionized or Distilled Water. 3. Shake The Bottle Vigorously to Completely Mix The Components of The Concentrate (The Solution May Separate Into Phases Over Time). 4. Store with Cap Tightly Secured. Protocol Recommendations 1. Deparaffinize and Rehydrate Tissue Sections. 2. Place Slides Into 1 x Retrieval Solution in a Slide Container (e.g. Coplin Jar, Tissue-Tek, Staining Dish or Metal Slide Canister). 3. Retrieve Sections Under Pressure 4. After Take-Off Reagent Jar Containing Slides From Pressure Cooker, Allow The Slides to Cool for 20 Minutes to Reach Room Temperature. 5. Wash Slides in Deionized Water and Then with Wash Buffer. Proceed with Immunostaining Recommendations in The Antibody Datasheet. 6. Gently Rinse by Gradually Adding DI Water to The Solution, Then Remove Slides and Rinse with DI Water. (1)
The Antigen Retrieval Protocol is Recommended for Use in Tissues That Have Been Fixed in Formalin Only. Ensure That The Fixed Sections Are Adequately Embedded in Paraffin. Cut Tissue Sections to 4-5 Microns. Preparation of Working Solutions 1. The 10 x Concentrated Format Should be Diluted Tenfold with Distilled or Deionized Water. 2. Mix One Part of Concentrated Antigen Retrieval Solution with Nine Parts of Deionized or Distilled Water. 3. Shake The Bottle Vigorously to Completely Mix The Components of The Concentrate (The Solution May Separate Into Phases Over Time). 4. Store with Cap Tightly Secured. Protocol Recommendations 1. Deparaffinize and Rehydrate Tissue Sections. 2. Place Slides Into 1 x Retrieval Solution in a Slide Container (e.g. Coplin Jar, Tissue-Tek, Staining Dish or Metal Slide Canister). 3. Retrieve Sections Under Pressure. 4. After Take-Off Reagent Jar Containing Slides From Pressure Cooker, Allow The Slides to Cool for 20 Minutes to Reach Room Temperature. 5. Wash Slides in Deionized Water and Then with Wash Buffer. Proceed with Immunostaining Recommendations in The Antibody Datasheet. 6. Gently Rinse by Gradually Adding DI Water to The Solution, Then Remove Slides and Rinse with DI Water. (3)
Transient transfection for mammalian AAV production (3)
Use in functional reported in scientific literature (PMID: 24371146) (1)
Used as Anti-coagulant/Blood preservative (1)
Used for binding, washing, elution of IgG according to recommended protocols, purification of Monoclonal and Polyclonal IgG using immobilized Protein A or Protein G (1)
Used for dilution of calibrators and samples for drug abuse assays (1)
Used in formulating animal research diets (1)
Western Blot (2)
Wright Staining (2)