Western Blot Substrates and Substrate Kits
Novus Biologicals™ UltraScence Pico Plus Western Substrate
Luminol-based enhanced chemiluminescent substrate
Novus Biologicals™ UltraScence Femto Plus Western Substrate
Luminol-based enhanced chemiluminescent substrate
Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution
Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.
Novus Biologicals™ UltraScence Pico Ultra Western Substrate
Luminol-based enhanced chemiluminescent substrate
Thermo Scientific™ Pierce™ CN/DAB Substrate Kit
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
MilliporeSigma™ Calbiochem™ TMB, Insoluble
Peroxidase substrate which produces stable, insoluble, aquamarine blue precipitate at reaction site.
Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate)
This CDP-Star™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.
Molecular Probes™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)
The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.
Seracare Life Sciences Inc TMB 1-C MEMBRANE HRP SUBSTRATE
Most sensitive chromogenic substrate for peroxidase detection. It develops a deep blue color at the reaction site in the presence of peroxidase-labeled conjugates. Recommended for membrane assays and dot blots. 1-component format of 3, 3', 5, 5' - Tetramethylbenzidine, no reagent preparation, hence, very convenient.
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST BCIP/NBT
Synonym: BCIP/NBT Alkaline Phosphatase Substrate. Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.
Genetex Inc Trident femto Western HRP Substrate
GeneTex Trident femto Western HRP Substrate can detect horseradish peroxidase at the femtogram level. The substrate is supplied as two components.
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Cell Signaling Technology SignalFire Elite ECL Reagent | 20ml
SignalFire Elite ECL Reagent from Cell Signaling Technology (CST) is an ultra sensitive chemiluminescent substrate capable of detecting femtogram amounts of protein by western blot analysis. SignalFire™ Elite ECL Reagent is compatible with both film and digital imaging systems. The extremely intense signal output allows detection of very low abundance proteins, conservation of reagents, and short exposure times.