Western Blot Substrates and Substrate Kits
Seracare Life Sciences Inc PHOSPHAGLORESERVE AP SUBSTRATE
PhosphaGLO Reserve AP Substrate is the chemiluminescent substrate of choice when greatest senstivity is required for detection of alkaline-phosphatase (AP) in Western blotting or ELISA. Product is a ready-to-use, 1-component substrate requiring no special blocking solutions. Other features and benefits include: Excellent sensitivity; detect femotogram levels of protein. Long glow times up to five days; allows for repeated exposures (Western Blot) or luminosity readings (ELISA). Easy-to-follow protocol; simply add to microwell or blot and detect within minutes. Product is stable for two years at 4deg. C; no worries about rapidly degrading substrates. Compatible with film or chemiluminescent imager; provides flexibility for recording permanent results.
FUJIFILM BIOSCIENCES INC SCALEVIEW S Trial Kit
SCALEVIEW-S Trial Kit consists of tissue clearing reagents used for ScaleS a tissue clearing method developed by Dr Atsushi Miyawaki and his colleagues This method uses solutions with mainly urea The tissue is made transparent and adjusted RI for microscopic observation by soaking in 6 kinds of solutions SCALEVIEW-S0 SCALEVIEW-S1 SCALEVIEW-S2 SCALEVIEW-S3 SCALEVIEW-S4 SCALEVIEW-SMt 2mm section can be made transparent in 1 day using the reagents The method can make a transparent tissue keeping the structure of proteins Therefore it is possible to observe the tissue expressing fluorescent proteins and to label by antibody and/or fluorescent dye after tissue clearing Its also applicable to not only mouse and rat brain but also human after dye The transparent tissue can be observed for electric microscope or confocal microscope
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Genhunter Corporation PerfectWestern, Large Rainbow Set of 6 colors
PerfectWestern, Large, Rainbow Set (Qty. 1-4 sets)
Genetex Inc Trident pico Western HRP Substrate
Trident pico Western HRP Substrate can detect horseradish peroxidase at the low pico to mid femtogram level. Trident pico Western HRP Substrate provides superior sensitivity compared to competitor products and is more economical. The substrate is supplied as two components. Substrate may be used for any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Detection and analysis may be done by CCD imaging systems or x-ray film.
Abcam ECL Western Blotting Substrate Kit, 50 Tests
The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots.
The product is subject to the following: Abcam Restricted Use Statement
Abcam ECL Substrate Kit (High Sensitivity), 200ML
High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.
The product is subject to the following: Abcam Restricted Use Statement
Biotium DNA Binding Dyes DNA Gel Stains and Accessories DNA Gel Stains Chemical Reagents 1/EA
The EMBER Ultra DNA Gel Kit is optimized for superior DNA sensitivity and resolution using ultra-sensitive EMBER dye The kit includes EMBER Ultra Precoated Agarose and EMBER Ultra DNA Loading Dye Ideal for blue LED imagers also compatible with UV gel boxes Trial size kit makes approximately 10 minigels
Seracare Life Sciences Inc LUMIGLO RES HRP CHEMI SUBS
LumiGLO Reserve is a lumino-based chemiluminescent substrate designed for rapid and sensitive detection of peroxidase-labeled conjugates. This substrate provides maximum sensitivity in the femtogram range, making it ideal for applications where the protein of interest is in low concentrations. Given the high light intensity, images may be more quickly captured and multiple exposures easily obtained. The 2-component format is easily prepared and is provided with KPL's Wash Solution, futher enabling blots with excellent signal-to-noise.