ELISA Substrates and Reagents
Neogen Diagnostics TMB Membrane Substrate, 100 ML, HRP, Membrane, Colorimetric
TMB Membrane Substrate is a ready to use solution. It contains 3,3’,5,5’-tetramethyl-benzidine in a mildly acidic buffer in a one bottle format. TMB Membrane Substrate develops an insoluble, permanent dark blue reaction in the presence of peroxidase. The substrate is intended for western blot, dot blots, and line assays and offers comparable performance and sensitivity to some chemiluminescent substrates. This substrate is not recommended for microwell or immunohistochemical applications. Stable for 2 years, from the date of manufacture, when stored at room temperature (15 to 25C) or 48 months when stored at 2-8C.
Surmodics Inc TMB Double Slow One Component HRP Microwell Substrate, 1L
BioFX™ TMB Double Slow Kinetic One Component HRP Microwell Substrate is a low-kinetic range substrate for ELISA applications.
Neogen Diagnostics Enhanced K-Blue TMB Substrate, 500 mL, Microwell, HRP, Colorimetric
Enhanced K-Blue is a one bottle stabilized chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Enhanced K-Blue develops a deep blue color in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Stable for a minimum of 48 months when stored at 2-8C.
Surmodics Inc TMB Super Slow One Component HRP Microwell Substrate, 1L
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
Biolegend Purified anti-PRAS40/AKT1S1
Purified anti-PRAS40/AKT1S1 [W22030A]; Isotype: Rat IgG2a, κ; Reactivity: Human, Mouse, Rat; Apps: WB, ICC; Size: 25 μg
Kingfisher Biotech Inc Swine IL-6 ELISpot - Up to two (2) ELISpot assays
Swine IL-6 ELISpot contains Capture Antibody, Standard Control, and Detection Antibody for development of up to two (2) ELISpot assays. Other required materials and reagents are not supplied. The antibodies have been determined to function in an ELISpot with the Standard Control provided. Components may also be purchased separately. For research use only. Made in the USA
Biolegend Purified anti-PRAS40/AKT1S1
Purified anti-PRAS40/AKT1S1 [W22030A]; Isotype: Rat IgG2a, κ; Reactivity: Human, Mouse, Rat; Apps: WB, ICC; Size: 100 μg
Revvity Health Sciences Inc HTRF Lysis & Detection Buffer #8, 200 mL
HTRF Lysis & Detection Buffer #8, 200 mL
Cayman Chemical 4-ANPP-d5 (exempt preparation), Purity: ≥99% deuterated forms (d1-d5), CAS: 1189466-15-6, 1 mg
An internal standard for the quantification of 4-ANPP by GC- or LC-MS; provided as a DEA exempt preparation; intended for research and forensic applications
Kingfisher Biotech Inc Swine IL-1 Receptor Antagonist ELISpot - Up to two (2) ELISpot assays
Swine IL-1 Receptor Antagonist ELISpot contains Capture Antibody, Standard Control, and Detection Antibody for development of up to two (2) ELISpot assays. Other required materials and reagents are not supplied. The antibodies have been determined to function in an ELISpot with the Standard Control provided. Components may also be purchased separately. For research use only. Made in the USA
Kingfisher Biotech Inc Rabbit IFN gamma ELISpot - Up to two (2) ELISpot assays
Rabbit IFN gamma ELISpot contains Capture Antibody, Standard Control, and Detection Antibody for development of up to two (2) ELISpot assays. Other required materials and reagents are not supplied. The antibodies have been determined to function in an ELISpot with the Standard Control provided. Components may also be purchased separately. For research use only. Made in the USA
Biotium Glycophorin A / CD235a (Erythrocyte Marker) (GYPA/1725R), CF740 conjugate, 0.1mg/mL
This antibody recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non