ELISA Substrates and Reagents
ABclonal Technology Phospho-CDK9-T186 Rabbit pAb
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
ABclonal Technology Phospho-NDRG1-S330 Rabbit pAb
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ABclonal Technology Phospho-SMC1-S957 Rabbit pAb
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
ABclonal Technology ERK1 / ERK2 Rabbit pAb
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
ABclonal Technology Phospho-eIF2α-S51 Rabbit pAb
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
ABclonal Technology Phospho-BRD4-T249 Rabbit pAb
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Electron Microscopy Sciences TMB 3,3'5,5'-Tetramethylbenzidine 1 GR
C₁₆H₂₀N₂ F.W. 240.34
Assay: >98%
Melting point: 166-169°C
A non-carcinogenic substitute for DAB and DAB-HCl. Used as a sensitive and specific reagent for the detection of blood. Standefer, J.C., Vanderjagt, D. (1977) Assay of Hemoglobin. Clin. Chem. 23, 749, Boss, E.S., et al., (1981). Assay of Peroxidases. J. of Immunoassay. 2,187. and Holland, V.R., et al., (1974) Tetrahedron, 30, 3299.
American Research Products Inc Mouse WAP four disulfide core
Mouse WAP four-disulfide core domain protein 2 (WFDC2) ELISA kit from CUSABIO Detection Range 62 5 pg/mL-4000 pg/mL Sensitivity 15 6 pg/mL Sample serum plasma tissue homogenates Method Sandwich
ABclonal Technology Fetuin A/AHSG Rabbit pAb
The protein encoded by this gene is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including endocytosis, brain development, and the formation of bone tissue. Defects in this gene are a cause of susceptibility to leanness.
MilliporeSigma BCIP/NBT SINGLE REAGENT
BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol)
Abcam TMB ELISA Substrate (Slow Kinetic Rate), 1000ML
Abcam's TMB ELISA Substrate (Slow Kinetic Rate) detects horseradish peroxidase (HRP) activity and contains 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer.
The product is subject to the following: Abcam Restricted Use Statement