ELISA Substrates and Reagents
ABclonal Technology Phospho-IRE1-S724 Rabbit pAb
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
ABclonal Technology Phospho-NF-kB p65/RelA-T435 Rabbit pAb
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ABclonal Technology Decorin Rabbit pAb
This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients.
ABclonal Technology Phospho-SHP2-Y542 Rabbit pAb
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
ABclonal Technology ERK1 / ERK2 Rabbit pAb
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
ABclonal Technology Phospho-STK3/MST2-T384 Rabbit pAb
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Abcam 450 nm Stop Solution for TMB Substrate, 100ML
Abcam's 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection.
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology Phospho-MEF2C-S396 Rabbit pAb
This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe cognitive disability, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described.
ABclonal Technology Phospho-AKT1-S473 Rabbit pAb
This gene encodes a member of the AKT serine-threonine kinase family (AKT1), which is involved in cell signaling. AKT proteins contain a pleckstrin homology domain, kinase domain, and a regulatory domain. They are phosphorylated by PI3K and play a key role in regulating cell proliferation, survival, metabolism, and angiogenesis. Activated by phosphatidylinositol 3,4,5-trisphosphate (PIP3), AKT proteins regulate numerous cellular functions and are critical for tumor cell survival, inhibiting apoptosis and regulating the mTOR pathway. AKT1 mutations are associated with cancer types, including breast, colorectal, and ovarian cancers, as well as disorders like Proteus syndrome and Cowden syndrome. Multiple spliced variants of this gene exist.
ABclonal Technology Phospho-Tyk2-Y1054/1055 Rabbit pAb
This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also a component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with Immunodeficiency 35.
ABclonal Technology Phospho-BRD4-S1070 Rabbit pAb
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
ABclonal Technology Phospho-MET-Y1234/1235 Rabbit pAb
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.
ABclonal Technology Phospho-NRF2-S40 Rabbit pAb
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters, many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
ABclonal Technology Phospho-PPP1R12A-S507 Rabbit pAb
Myosin phosphatase target subunit 1 (MBS) is a key component of myosin phosphatase, which regulates actin-myosin interactions downstream of the Rho GTPase. RhoA, when active in its GTP-bound form, interacts with MBS to control myosin light chain (MLC) phosphorylation, influencing smooth muscle contraction and nonmuscle cell movement. Rho-kinase, activated by GTP-bound RhoA, phosphorylates and inactivates MBS, reducing myosin phosphatase activity. Overexpression of RhoA or activated RhoA increases MLC phosphorylation. Several transcript variants encoding different isoforms of this gene have been identified.