Electrophoresis Gel Stains
Bio Rad Laboratories COOMASSIE BRILLIANT BLUE G-250
Coomassie Brilliant Blue G-250 protein stain powder, 10g
Biotium DNAzure Blue Nucleic Acid Gel Stain, 100X
DNAzure Blue Gel Stain allows you to visualize DNA bands in gels by eye, with detection sensitivity rivaling most fluorescence-based gel stains. Key to the technology is a DNA-binding dye that turns from colorless to deep blue upon exposure to light. The benefits include: deep blue bands visible by the naked eye following 5-30 min. light exposure, ultrasensitive detection (as little as ~1 ng DNA), and simplified DNA band excision without DNA-damaging UV Light.
Biotang Inc PYRONIN Y (10MG/ML) 10 ML, BP-113, 10mg/ml Pyronin Y solution prepared in 18.2 megohms water and filtered through 0.22 micron filter.
PYRONIN Y (10MG/ML) 10 ML, BP-113, 10mg/ml Pyronin Y solution prepared in 18.2 megohms water and filtered through 0.22 micron filter.
Alkali Scientific SmartGlow™ Safe Green Pre Nucleic Acid Stain, Loading Dye, 1mL
SmartGlow Nucleic Acid Stain is ideal for excitation of nucleic acids with UV or visible blue light transilluminators. Unlike EtBR, SmartGlow it is non-mutagenic and does not require special methods of disposal. SmartGlow PS (Pre Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA, ssDNA and RNA. SmartGlow PS exhibits excitation peaks at 290nm and 490nm, allowing it to be used with UV and blue light. SmartGlow LD (Loading Dye) is supplied at 6X concentration and is added directly to the samples. No dye needs to be added to the gel or running buffer. After electrophoretic separation, view and document your results using a UV or blue light illuminator. SmartGlow LD emits green fluorescence when bound to dsDNA, ssDNA and RNA. Replaces hazardous EtBr Excitation by UV or blue light Non-mutagenic, environmentally safe Two types: PS (Pre-Stain) LD (Loading Dye)
Sigma Aldrich Fine Chemicals Biosciences EZBlueTM Gel Staining Reag
Convenient sensitive and safe EZBlue(TM) Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain it reacts only with proteins not the gel itself. Background staining is reduced so protein bands can be visualized almost immediately. No destaining step is required although a water wash may intensify bands and clarify the background. Most impressively EZBlue is extremely sensitive detecting as little as 5 ng of protein.
Biotium GelGreen Nucleic Acid Gel Stain, 10, 000X in Water, 10 mL
GelGreen is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen is far more sensitive than SYBR Safe. Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally. GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as blue LED light box, 488 nm laser-based gel scanner, or Dark Reader). GelGreen can be used for either post-electrophoresis gel staining or precast gel staining. Properties: Liquid; Store at room temperature and protect from light.
Biotium NucSpot 470, 1000X in DMSO
NucSpot 470 is a cell membrane-impermeant green fluorescent DNA stain. It is virtually non-fluorescent in the absence of DNA, but fluoresces bright green upon DNA binding. While other green nucleic acid stains like TOTO, TO-PRO, or SYTOX dyes stain both the nucleus and cytoplasm, NucSpot 470 specifically stains the nucleus of fixed and permeabilized cells. It also can be used to selectively stain dead cells in living cultures. NucSpot 470 has an excitation/emission of 460/546 nm and can be imaged using standard settings for FITC. Properties: Ex/Em= 460/546 nm (with DNA); Store at 4°C and protect from light.
Biotium Hoechst 33258, 10mg/mL in H2O
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Hoechst 33528 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required. Properties: Yellow solution; Store at 4°C, protect from light; Ex/Em= 352/461 nm (with DNA); C25H27Cl3N6O; MW: 533.88; [23491-45-4].
Biotium PAGE GelRed Nucleic Acid Gel Stain, 10,000X in Water
PAGE GelRed is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. PAGE GelRed can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. While PAGE GelRed also can be used to stain DNA in agarose gels, Biotium’s original GelRed nucleic acid gel stain is more sensitive for agarose gel applications. PAGE GelRed can be removed from DNA after agarose gel staining using commonly available gel extraction kits. PAGE GelRed is 6-8 times more sensitive for dsDNA over RNA. Properties: Liquid; Ex/Em= 308, 551/625 nm (with dsDNA); Store at room temperature and protect from light.
Fisher BioReagents™ EZ-Run™ Protein Gel Staining Solution
Ready-to-use solution for highly sensitive staining of proteins separated in polyacrylamide gels
MilliporeSigma™ Calbiochem™ OmniPur™ Ethidium Bromide
Suitable for fluorescent staining of nucleic acids.
MilliporeSigma™ OmniPur™ Fast Green FCF
For Protein Staining
Novus Biologicals™ InstantBlue Protein Stain
InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels.
MP Biomedicals™ BBXG (With Glycerol)
Used as a loading dye for DNA samples. MP Biomedicals BBXG (With Glycerol) is a 10x solution of Bromophenol Blue, Xylene Cyanol FF and Glycerol that is used as a loading buffer for DNA gel electrophoresis.