Electrophoresis Gel Stains
Biotium Nucleic Acid Gel Stain, 10,000X in Water; Trial size (0.1 mL)
GelRed is a sensitive, stable and environmentally safer alternative to the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose or polyacrylamide gels (see PAGE GelRed Nucleic Acid Gel Stain). The dye is noncytotoxic, nonmutagenic and nonhazardous at concentrations used in gel staining. It can be conveniently disposed down the drain or in regular trash at working concentrations, reducing disposal costs. It is much more sensitive than EB or SYBR Safe and extremely stable for long-term room temperature storage. Simple to use in precast or post-electrophoresis gel staining and detected with a UV transilluminator in the same optical setting used for EB. Compatible with downstream gel purification, restriction digest, sequencing and cloning. See also GelGreen Nucleic Acid Gel Stain, the safer replacement for SYBR gel stains.
Newcomer Supply Staining Rack for 20 Slide Staining Dish - Stainless Steel
The Stainless Steel Staining Rack accommodates 20 horizontal slides. Outside Dimensions: 3 3/8" x 2 1/2" x 2 1/8" (H). Racks are also available in 2 other sizes to accommodate 30 and 60 slides.
Bio Rad Laboratories COOMASIE BR.BLUE R250 10G
Coomassie Brilliant Blue R-250 Reagent, 10g, The fastest and easiest way to Coomassie-stain Criterion or other protein polyacrylamide gels
Biotium DAPI, 10mg/mL in H2O
DAPI (4',6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H2O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate solid, which is more water soluble than the dihydrochloride form of the dye. Classic blue nuclear counterstain for fixed cells Can be used at higher concentrations to stain live cells &lamba;Ex/&lamba;Em (with DNA) = 358/461 nm Yellow solution Store at 4°C and protect from light C22H27N5O6 MW: 457.49
Biotium PMA 1MG
PMA (propidium monoazide) is a high affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. It preferentially binds to dsDNA with high affinity. Upon photolysis, the dye covalently reacts with DNA, thus resulting in permanent DNA modification. The dye is cell membrane-impermeable, and thus can be selectively used to modify only exposed DNA from dead cells while leaving DNA from viable cells intact. This feature allows selective detection of viable pathogenic cells by quantitative real-time PCR in the presence dead cells whose DNA has been PMA-modified and thus can not be amplified. PMA has been used extensively in bacterial detection related to food and water safety, medical diagnosis and biodefense. 1 mg Molecular weight: 511 Abs = 464 nm (before photolysis) Abs/Em = 510/610 nm (following reaction with DNA/RNA) Dark red solid soluble in DMSO or DMF Store at 4�C and protect from light at all times
Bio Rad Laboratories DC PROTEIN ASSAY REAGENT A
The protein assay reagent A, alkaline copper tartrate solution, is used in the DC protein assay, which quantitates proteins following detergent solubilization. Supplied as 250 ml alkaline copper tartrate. (500-0113)
Biotium EvaGreen Dye, 2000X in DMSO, 50uL
EvaGreen dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.
Cayman Chemical STI2 (Bio-Rad) Multiplexed qPCR Assay, 1 ea
Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes: • Tube 1: 5X Primers and probes for 4 pathogens and human control • Tube 2: Synthetic positive controls • Tube 3: Spike-in RPP30 control • Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria (no cell well), Multiplexed Reaction Panels: STI, Vaginal microbiota Colors: Cy5 (Mycoplasma genitalium), Cy5.5 (Ureaplasma urealyticum), FAM (Neisseria gonorrhoeae), HEX (RPP30), TEX615 (Treponema pallidum) Instruments: Bio-Rad CFX96 or CFX384
Cayman Chemical STI4 (Bio-Rad) Multiplexed qPCR Assay, 1 ea
Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 4 tubes: • Tube 1: 5X Primers and probes for 4 pathogens and human control • Tube 2: Synthetic positive controls • Tube 3: Spike-in RPP30 control • Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Virus dsDNA, Multiplexed Reaction Panels: STI, Vaginal microbiota Colors: Cy5 (Chlamydia trachomatis), FAM (HSV-1 and HSV-2), HEX (RPP30), TEX615 (Neisseria gonorrhoeae) Instruments: Bio-Rad CFX96 or CFX384
LICORbio Odyssey, Bis-Tris, 10x8, 10%, 10 wells (pack of 10)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
Crescent Chemical Co Inc COOMASSIE BRILLIANT BLUE
Coomassie Brilliant Blue G 250 Corresponds to SERVA Blue G (cat. no. 35050). Size - 25G Storage Conditions - +15 C TO +30 C Catalog Number - 17524.01 CAS 6104-58-1
LICORbio Odyssey, Bis-Tris, 10x8, 12%, 15 wells (pack of 10)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.