Gel Electrophoresis Reagents and Buffers

Invitrogen™ Novex™ Sharp Pre-stained Protein Standard

Designed for accurate, easy, and convenient molecular weight estimation of a wide range of molecular weight proteins during SDS-PAGE and Western blotting

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BioAcryl-P (40%, 19:1)

BioAcryl-P (Acrylamide/Bisacrylamide 29:1, CAS # 25034-58-6) is a powder-based formula used to create the gel matrix necessary for electrophoresis experiments.

Thermo Scientific Chemicals Acetate Buffer (1 M, pH 5.5)

CAS: 127-09-3

R&D Systems™ Sample Diluent Concentrate 2 (2X)

Contains 2% NP-40 Alternative, 40mM Tris, 274mM NaCl, 20% glycerol, 2mM EDTA, and 2mM activated sodium orthovanadate

Thermo Scientific Chemicals Glycine, 0.2M buffer soln., pH 2.5, low endotoxin

CAS: 56-40-6 Molecular Weight (g/mol): 75.067

R&D Systems™ Reagent Diluent Concentrate 1

Concentrated buffered protein base

Thermo Scientific Chemicals SM buffer, pH 7.5

Thermo Scientific Chemicals Acrylamide/Bisacrylamide Solution (40%, 37.5:1)

Acrylamide/Bisacrylamide Solution (40%, 37.5:1), also known as bioAcryl-P (40%, 37.5:1), is a premixed solution containing acrylamide and N,N'-methylenebisacrylamide (in a ratio of 37.5:1, respectively) used for preparing protein DNA gels.

Thermo Scientific Chemicals Acetate Buffer (1 M, pH 3.0)

CAS: 127-09-3

Agarose, Electrophoresis Grade, MP Biomedicals™

CAS: 9012-36-6 MDL Number: MFCD00081294 Synonym: 3, 6-Anhydro-α-L-galacto-β-D-galactan,FastLane agarose

Thermo Scientific Chemicals Citrate, 0.5M buffer soln., pH 5.5

CAS: 4-3-6132 Molecular Weight (g/mol): 294.10

Silica gel 60, 0.019-0.037mm (400-600 mesh)

CAS: 7631-86-9 Molecular Formula: O2Si Molecular Weight (g/mol): 60.08 MDL Number: MFCD00011232 MFCD00217788 MFCD00163736 MFCD00148266 MFCD00603035 MFCD02100519 MFCD06202255 MFCD07370733 InChI Key: VYPSYNLAJGMNEJ-UHFFFAOYSA-N PubChem CID: 24261 ChEBI: CHEBI:30563 IUPAC Name: silanedione SMILES: O=[Si]=O

Thermo Scientific Chemicals Agarose Gel Loading Dye (6X, Alkaline)

Silica gel, C18-RP, 17%C, approx. 0.7 mmol/g, part. size: 48-65 micron

CAS: 71889-02-6 Molecular Formula: C18H37Cl3O2Si2 Molecular Weight (g/mol): 448.012 InChI Key: VYTRPUAMVCWPLO-UHFFFAOYSA-N Synonym: Octadecylsilicate PubChem CID: 172932 IUPAC Name: dioxosilane;trichloro(octadecyl)silane SMILES: CCCCCCCCCCCCCCCCCC[Si](Cl)(Cl)Cl.O=[Si]=O

Thermo Scientific Chemicals Phosphate, 0.2M buffer soln., pH 4.4

CAS: 7558-80-7 Molecular Weight (g/mol): 119.976