Sodium Citrate Buffer, 0.5M, pH 5.0 is a commonly used buffer composed of citric acid and sodium citrate, widely utilized in molecular biology, immunohistochemistry (IHC), and biochemistry. It serves various critical functions in laboratory settings.
- Used in heat-induced epitope retrieval (HIER) to reverse antigenicity loss in formalin-fixed paraffin-embedded tissues.
- Can be applied as an RT-PCR enhancement reagent.
- Utilized as a vehicle for Captisol in animal tumor model studies.