Tissue Fixatives
Scigen O.C.T. Compound Cryostat Embedding Medium
Provides convenient specimen matrix for cryostat sectioning at temperatures of -10°C and below. It is a formulation of 10.24% polyvinyl alcohol, 4.26% polyethylene glycol and 85.5% nonreactive ingredients, being water soluble; it leaves no residue during staining.
Electron Microscopy Sciences R-Buffer B (10x), 250 ml for use in Retriever Thermal Slide Processor 250 ML
Used with the Retriever benchtop thermal slide processer that is used on formalin-fixed, paraffin embedded tissues prior to immunostaining.
Electron Microscopy Sciences EMS Xylene Substitute 1 GL
The xylene substitute is for use in histology and cytology. It is safer and a more preferable alternative to xylene and cuases minimal tissue shrinkage. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives. Dries slowly with no residue. It can be used in all procedures that require xylene and is less toxic than xylene. Soluble with alcohol, paraffin embedding media, and mounting media. Biodegradable.
Electron Microscopy Sciences EMS Hemo-De Xylene Substitute 1 GL
The xylene substitute is for use in histology and cytology. It is safer and a more preferable alternative to xylene and cuases minimal tissue shrinkage. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives. Dries slowly with no residue. It can be used in all procedures that require xylene and is less toxic than xylene. Soluble with alcohol, paraffin embedding media, and mounting media. Biodegradable.
Electron Microscopy Sciences EMS Hemo-De Xylene Substitute 5 GL
The xylene substitute is for use in histology and cytology. It is safer and a more preferable alternative to xylene and cuases minimal tissue shrinkage. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives. Dries slowly with no residue. It can be used in all procedures that require xylene and is less toxic than xylene. Soluble with alcohol, paraffin embedding media, and mounting media. Biodegradable.
Bioworld In Situ™ Tissue Fixative, 500 mL
In SituT Fixative is utilized for routine, manual or automated tissue fixation. Cellular material or tissues can be immersed in In Situ for transport and preservation of antigenic sites. In Situ maintains the morphological integrity of cells or tissues, and enhances specific immunohistochermical staining in tissues embedded in paraffin medium.• In Situ permits in situ PCR reactions.• A nonhazardous tissue fixative exempt from EPA disposal regulations.• Virtually odorless.• Inactivates the HIV-1 virus in culture.• Maintains cellular morphology.• Optimizes immunohistochemistry results.
Bioworld In Situ™ Tissue Fixative, 100 mL
In SituT Fixative is utilized for routine, manual or automated tissue fixation. Cellular material or tissues can be immersed in In Situ for transport and preservation of antigenic sites. In Situ maintains the morphological integrity of cells or tissues, and enhances specific immunohistochermical staining in tissues embedded in paraffin medium.• In Situ permits in situ PCR reactions.• A nonhazardous tissue fixative exempt from EPA disposal regulations.• Virtually odorless.• Inactivates the HIV-1 virus in culture.• Maintains cellular morphology.• Optimizes immunohistochemistry results.
Electron Microscopy Sciences Hard Plus Resin 812 Kit
Hard-Plus Resin-812 is a newly formulated resin that enables the processing of specimens that have a tendency to wrinkle and be problematic. WPE numbers do not vary much from batch to batch, there is good stability under the electron beam, and it stains well with heavy metals. Viscosity is lower than original Epon-812, making infiltration of tissue more complete.
For processing and embedding, it can be used same way as Epon-812, but it is not necessary to use intermediate solvents and infiltration can immediately follow with absolute alcohol.
Polymerization normally at 60 degree C for 2448 hrs. Longer times or higher temps (+70 degree C) will give harder block. For larger samples (>1x1 mm3) use of intermediate solvent recommended. Supplied only as a premix kit. Each kit contains five separate mini-kits, making approximately 100g each.
Includes: 5x50g Hard-Plus Resin-812, 5 x 50g Hardener Hard-Plus, 5 x 2.5 ml Accelerator