PCR Equipment and Supplies
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Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Purification Time | 7 min. |
| Starting Material | 1-10 Cells |
| For Use With (Application) | RT-PCR |
| PCR Method | 2-step RT-qPCR |
| Product Line | Ambion, Cells-to-CT |
| Detection Method | TaqMan |
Thermo Scientific™ dsDNase
dsDNase is a heat inactivatable recombinant shrimp DNase used to remove genomic DNA contamination of RNA samples before reverse transcription.
Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - BRAF 476
Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | SYBR |
| Detection Method | SYBR |
Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | Direct PCR |
| Polymerase | Phusion Blood II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ Platinum™ Tfi Exo(-) DNA Polymerase
Platinum™ Tfi Exo(-) DNA Polymerase
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ M13 Reverse Primer
Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Reverse Transcriptase | M-MLV |
|---|---|
| Form | Liquid |
| InhibitorToleranceSimple | High |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | Not Applicable |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
| Sample Type | RNA, cDNA, DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Built-In Hot Start |
| Product Line | TaqPath |
Invitrogen™ Biotin-14-dATP
A patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Purity or Quality Grade | UP (Ultra Pure) |
|---|---|
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
| Sample Type | Environmental Sample, Food |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Product Line | TaqMan |
Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Form | Liquid |
| Gene Symbol | ACTB |
| Amplicon Size | 171 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | NM_001101.2 |
| Target | β-actin |
| Format | Tube |
| Label or Dye | FAM |
| Species | Human |
| Product Line | FAM, TaqMan |
Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Form | Liquid |
| Gene Symbol | GAPDH |
| Amplicon Size | 122 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | NM_002046.3 |
| Target | GAPDH |
| Format | Tube |
| Label or Dye | FAM |
| Species | Human |
| Product Line | FAM, TaqMan |