Oligonucleotides
Abcam alpha-NETA, Fluorescent ChAT inhibitor, 5MG
MW 369.24 Da. alpha-NETA, Fluorescent ChAT inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Fludarabine (FAMP), DNA synthesis inhibitor, 50MG
MW 285.23 Da, Purity >98%. DNA synthesis inhibitor. Prodrug converted to active metabolite F-ara-A which inhibits ribonucleotide reductase and competitively inhibits DNA polymerase. Also prevents DNA ligase I from joining DNA. Induces apoptosis. Active in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences X tremeGENETM HP DNA Trans
X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA then transports the complex into animal or insect cells.
Sino Biological Human GAPDH qPCR Primer Pair
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Cell Biologics Human Primary Pulmonary Vein Endothelial Cells
Human Primary Pulmonary Vein Endothelial Cells
INTACT GENOMICS INC igScript™ Probe-Based One Step RT-qPCR Kit 1000 Reactions
IgScript™ probe-based RT-qPCR kit combines i) IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix for high efficiency, wide dynamic range, sensitivity, and specificity. The cDNA kit includes 5x IgScript™ master mix (Reverse Transcriptase, RNase inhibitor, dNTPs, buffer, MgCl₂, stabilizers), plus oligo-dT [d(T)23VN] and random hexamer primers for complete coverage of mRNA and non-polyadenylated RNAs. IgScript™ Reverse Transcriptase is a recombinant MMLV enzyme with reduced RNase H activity and higher thermostability, enabling full-length cDNA synthesis at 42–50 ºC. The qPCR 2x master mix (excluding primers/template) contains proprietary buffer, hot start Taq polymerase, low ROX dye, MgCl₂, dNTPs, stabilizers, and enhancers for robust amplification.Applications: Gene expression validation, multiplexing, mutation detection, pathogen/viral detection, GMO characterization, profiling.
New England Biolabs, Inc. E. coli tRNA - 1 mg
E. coli tRNA is a mix of tRNAs isolated from E. coli and purified by chromatography to remove contaminating proteins, including nucleases, as well as other nucleic acids. This tRNA formulation sustains translation in E. coli-based in vitro protein synthesis reactions (also referred to as cell-free protein synthesis or CFPS). It is also suited for molecular biology applications that require highly pure and active prokaryotic tRNA.
- Highly active tRNA for in vitro translation
- Purified by chromatography, no organic solvents
- Highly pure with undetectable levels of nucleases and proteases
- Manufactured with reduced environmental impact
- Compatible with a variety of molecular biology workflows
New England Biolabs, Inc. Monarch® Spin Plasmid Miniprep Kit - 50 preps
The Monarch Spin Plasmid Miniprep kit, a newer version of our original Monarch Miniprep kit, offers a unique method for plasmid preparation and purification, enabling extraction of highly concentrated plasmid DNA from various bacterial strains, with yields of up to 20 ug of high-quality DNA. This kit features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 30 ul, for highly concentrated DNA in downstream application such as sequencing, restriction digestion, transformation, transfection, and more. A colored buffer system helps indicate completion of key lysis and neutralization steps for an easy to follow workflow and shorter, faster protocols allow for reduced hands-on time and less spin time. Compared to leading suppliers, these kits use significantly less plastic through uniquely designed columns and bottles with plastic reduction while still maintaining structural integrity for centrifugation or vacuum manifolds and other steps
Active Motif Mouse Negative Control Primer Set 1
Mouse Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with mouse samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP samples from multiple mouse cell lines.
Sigma Aldrich Fine Chemicals Biosciences Uracil-DNA Glycosylase hea
Uracil-DNA Glycosylase heat-labile contains the equally named enzyme found in the marine bacterium BMTU 3346. Like the UNG from E. coli it hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA excising uracil and creating alkali-sensitive abasic sites in the DNA. These abasic sites can be hydrolyzed by endonuclease heat or alkali treatment. Depending on how the DNA is prepared Uracil-DNA Glycosylase can be used to achieve general site-specific or strand-specific U-DNA cleavage. This enzyme shows lower thermostability and is therefore easier to inactivate.Enzyme CharacteristicsThe BMTU 3346 enzyme is inactivated more quickly (2 minutes at 95C) than the corresponding enzyme from E. coli (10 minutes at 95C). It has also been reported that UNG from E. coli remains partially active leading to the degradation of the dU-containing PCR product. In contrast the heat-labile UNG does not degrade dU-PCR products within at least several hours of incubation at 2 to 8C. Theref
Cayman Chemical ComasIe Blue R-250 100g
A dye that is commonly used in laboratories to stain or quantify proteins; typically gives blue bands on a clear background with a sensitivity of 50-100 ng/band; displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1