Oligonucleotides
*OXFORD NANOPORE TECHNOLOGIES INC OXFORD NANOPORE TECHNOLOGIES INC
NC3957869 MINION GRIDION FLOW CELL DNA
Sigma Aldrich Fine Chemicals Biosciences Uracil-DNA Glycosylase hea
Uracil-DNA Glycosylase heat-labile contains the equally named enzyme found in the marine bacterium BMTU 3346. Like the UNG from E. coli it hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA excising uracil and creating alkali-sensitive abasic sites in the DNA. These abasic sites can be hydrolyzed by endonuclease heat or alkali treatment. Depending on how the DNA is prepared Uracil-DNA Glycosylase can be used to achieve general site-specific or strand-specific U-DNA cleavage. This enzyme shows lower thermostability and is therefore easier to inactivate.Enzyme CharacteristicsThe BMTU 3346 enzyme is inactivated more quickly (2 minutes at 95C) than the corresponding enzyme from E. coli (10 minutes at 95C). It has also been reported that UNG from E. coli remains partially active leading to the degradation of the dU-containing PCR product. In contrast the heat-labile UNG does not degrade dU-PCR products within at least several hours of incubation at 2 to 8C. Theref
Active Motif DNA Library Prep Kit, High Sensitivity, Compatible with Illumina Platforms, Kit
The Active Motif DNA Library Prep Kit is a high-sensitivity solution designed for preparing next-generation sequencing (NGS) libraries from double-stranded DNA for Illumina platforms. It ensures accurate and reproducible results, even with low input material, by using molecular identifiers to minimize PCR duplicates. This kit supports various applications, including genomic DNA-seq, and is compatible with multiple Illumina platforms, offering flexibility and enhanced unique alignment rates.
Sigma Aldrich Fine Chemicals Biosciences DIG DNA Labeling and Detec
We are committed to bringing you Greener Alternative Products which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.AThe DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Invivogen ODN 2006 (ODN 7909), 5 mg
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects.
New England Biolabs, Inc. Monarch Mag PCR & DNA Cleanup Kit (5 ug) - 400 preps
The Monarch Mag PCR & DNA Cleanup Kit (5 ug) enables rapid and reliable purification and concentration of up to 5 μg of DNA, including both single- and double-stranded forms, from common enzymatic reactions such as PCR, restriction digestion, ligation, reverse transcription, and rolling circle amplification (RCA). Using a streamlined bind-wash-elute workflow with silica-coated magnetic beads, the kit delivers highly pure, inhibitor-free DNA ready for immediate use in downstream applications such as in vitro transcription (IVT), sequencing, restriction digestion, library construction, labeling, and other enzymatic manipulations. The magnetic bead format offers flexibility and scalability, supporting both manual and automated workflows. Additional modified protocols support varying DNA input amounts and sample volumes and enable purification of oligos, gDNA, and RCA products. The kit includes magnetic beads, optimized buffers, and reagents, all compactly packaged.
Zymo Research Corporation Quick16S Plus Library Kit V3V4
TheQuick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPurebead-based clean-ups. Additional library quantification analysis such as TapeStationanalysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.
