Oligonucleotides
BIOHIPPO AAV-L7-Cre-WPRE-hGH-polyA
Recombinant adeno associated virus expressing Cre under the L7 promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-HypoCretin-Cre-WPRE-hGH-polyA
Recombinant adeno associated virus expressing Cre under the HypoCretin promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
New England Biolabs, Inc. Duplex DNase - 1000 units
An engineered double-strand specific DNA endonuclease, dsDNase preferentially degrades double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA. This specificity toward dsDNA allows the enzyme to degrade contaminating dsDNA while maintaining the integrity of single stranded DNA molecules such as primers and probes within PCR mixes, allowing for efficient decontamination of PCR master mixes. Moreover, dsDNase displays the unique property of cleaving the DNA strand of a DNA:RNA hybrid duplex.
New England Biolabs, Inc. NEBNext Enzymatic 5hmC-seq Kit 24 rxns
The NEBNext Enzymatic 5hmC-seq Kit specifically detects 5hmC sites. The kit includes NEBNext Ultra II library prep reagents, and 5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing determination of the precise location of individual 5mC and 5hmC sites. The kit includes the E5hmC-seq adaptor and index primers are supplied separately.
New England Biolabs, Inc. E. coli tRNA - 1 mg
E. coli tRNA is a mix of tRNAs isolated from E. coli and purified by chromatography to remove contaminating proteins, including nucleases, as well as other nucleic acids. This tRNA formulation sustains translation in E. coli-based in vitro protein synthesis reactions (also referred to as cell-free protein synthesis or CFPS). It is also suited for molecular biology applications that require highly pure and active prokaryotic tRNA.
- Highly active tRNA for in vitro translation
- Purified by chromatography, no organic solvents
- Highly pure with undetectable levels of nucleases and proteases
- Manufactured with reduced environmental impact
- Compatible with a variety of molecular biology workflows
Invivogen ODN 2006 (ODN 7909), 5 mg
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects.