PCR Supplies
Meridian Life Science Glycerol-Free Taq HS High, 20ML
Supplied as a blend of high‑performance glycerol‑free Taq DNA Polymerase with a Taq hot-start antibody and is suitable for difficult, multiplex PCR that require fast amplification, whilst maintaining both high specificity and sensitivity.Glycerol-Free Taq HS High Conc. is ideal for PCR kit manufactures and PCR molecular test facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS High Conc. allows for concentrated bulk production of PCR master mixes and the glycerol free format is useful when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, Tween free, 5,000U
AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. AS ONE Taq DNA Polymerase has both a 5 prime to 3 prime DNA polymerase and a 5 prime to 3 prime' exonuclease activity. The enzyme lacks a 3 prime to 5 prime exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A overhang, which makes the enzyme ideal for TA cloning. Included with Taq is Tween free 10x Ammonium Buffer.
INTACT GENOMICS INC T7 RNA Polymerase, 5,000 Units
Bacteriophage T7 RNA Polymerase (RNAP) is a highly processive DNA-dependent RNA polymerase that catalyzes the transcription of T7 genes. It unwinds double-stranded DNA to allow access to the coding strand for transcription initiation. This 99 kDa enzyme is designed to perform in vitro RNA synthesis from a cloned DNA sequence under T7 promoters. RNA produced using this enzyme is suitable for many applications in research and biotechnology.
- Useful for sgRNA and mRNA synthesis.
- Catalyzes in vitro RNA synthesis from DNA templates.
- Suitable for applications in mRNA therapeutics.
- Physical purity is ≥85% as assessed by SDS-PAGE.
- Free from detectable RNase, Endonuclease (nicking), and DNase activities.
- Purified recombinant enzyme expressed and purified from E. coli.
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 500U
Taq DNA Polymerase 5 U/ul glycerol free. Glycerol free is ideal for automation and free drying applications.
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 10,000U
Taq DNA Polymerase 5 U/ul glycerol free. Glycerol free is ideal for automation and free drying applications
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free 10x Standard Buffer, 1,000U
Taq DNA Polymerase 5 U/ul glycerol free 10x Standard Buffer. Glycerol free is ideal for automation and free drying applications.
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free 10x Combination Buffer, 500U
Taq DNA Polymerase 5 U/ul glycerol free 10x Combination Buffer. Glycerol free is ideal for automation and free drying applications
As One International Inc Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, Tween free, 2,500U
AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. AS ONE Taq DNA Polymerase has both a 5 prime to 3 prime DNA polymerase and a 5 prime to 3 prime' exonuclease activity. The enzyme lacks a 3 prime to 5 prime exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A overhang, which makes the enzyme ideal for TA cloning. Included with Taq is Tween free 10x Ammonium Buffer.
Apexbio Technology LLC HYPERFUS PLUS DNA POLYMER 100U
HYPERFUS PLUS DNA POLYMER 100U APEXBIO TECHNOLOGIES
As One International Inc Taq DNA Polymerase 1.1x Master Mix, 1.5 mM MgCl2 (final concentration), 5,000Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 1.1X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 45 uL of the 1.1X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Enzo Life Sciences AMPIGENE HS Taq Mix (1000Reactions)
AMPIGENE HS Taq Mix uses the latest developments in polymerase technology and buffer chemistry to optimize and increase PCR sensitivity. The AMPIGENE HS Taq Mix is a robust mix using hot-start technology to increase sensitivity for PCR applications including genotyping, multiplex PCR, screening, library construction, colony PCR and PCR direct from blood and urine. An optimized buffer system allows efficient amplification under fast and standard cycling conditions. The AMPIGENE HS Taq Mix is resistant to PCR inhibitors and suitable for direct PCR from unprocessed samples: bacterial culture, bacterial colonies, blood, and urine. Applications: PCR.
Meridian Life Science Taq HS DNA Polymerase, 100UL
Taq HS DNA Polymerase is an antibody-mediated hot-start DNA polymerase provided with an optimized buffer system containing dNTP, MgCl2, stabilizers, and PCR enhancers at optimal concentrations which eliminates the need for optimization. The DNA polymerase has been developed to give more robust amplification than other polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors, making it ideal for fast PCR reactions across a range of templates, without compromising specificity or yield.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer, Tween free, 5,000U
AS ONE HS Taq DNA Polymerase is a hot start polymerase designed to diminish the formation of non-specific priming events during reaction set up and the first ramp of thermal cycling. HS Taq features high specificity and sensitivity, greater yield compared to standard DNA polymerases. HS Taq enables the detection of low abundance tragets. HS Taq Polymerase includes Tween free 10x Ammonium Buffer.
Meridian Life Science Gly-Free T7 RNA Polymerase, 50UL
A high concentration DNA-dependent RNA Polymerase with very high specificity for the T7 phage double-stranded promoter sequence. The enzyme catalyzes the 5’-3’ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from the promoter and is the industry standard enzyme for in vitro transcription (IVT). It can efficiently incorporate standard or modified ribonucleoside triphosphates (NTPs) synthesizing large amounts of mRNA very quickly, making it an ideal enzyme for in vitro synthesis of labeled or unlabeled RNA for research, diagnostic or clinical applications. Applications including isothermal diagnostic methods, such as nucleic acid sequence-based amplification (NASBA), mRNA vaccine production, and RNA-based therapeutic and research applications, including miRNA and siRNA synthesis for RNA interference drugs, synthesis of sgRNA for the CRISPR/Cas9 system.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer and 10x Combination Buffer, 2,500U
AS ONE HS DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Polymerase DNA Polymerase into the reaction. HS Taq includes 10x Ammonium Buffer and 10x Combination Buffer