PCR Supplies
Enzo Life Sciences AMPIGENE qPCR 1-Step Probe Kit No-ROX (200Reactions)
AMPIGENE qPCR 1-Step Probe Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Probe Kits are designed for use on a variety of probes including: Taqma , Molecular Beacons, and Scorpion probes. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Probe Kits come with either Lo-ROX, Hi-ROX, or No-ROX concentration. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.
Enzo Life Sciences AMPIGENE qPCR 1-Step Green Kit Hi-ROX (1000Reactions)
AMPIGENE qPCR 1-Step Green Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Green Kits use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Green Kits come with either Lo-ROX or Hi-ROX concentration. For Lo-ROX, see ENZ-NUC108. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below. Applications: PCR.
Immunology Consultants anti-Canine Coronavirus Antibody (Mouse Clone #4A9) - Monoclonal
This mouse monoclonal antibody provides highly specific detection of Canine Coronavirus (CCoV) antigens, supporting sensitive measurement in ELISA, Western blotting, and antigen‑capture assays. Clone 4A9 recognizes a defined viral epitope, enabling precise detection in complex canine samples with minimal background. Its strong affinity and clean signal make it suitable for diagnostic assay development, surveillance studies, and research on coronavirus infection dynamics in dogs. MCCV‑40A‑4A9 delivers consistent lot‑to‑lot performance, ensuring reliable antigen detection across repeated assays and diverse sample types.
Apexbio Technology LLC 1STEP COMP CELL PREP KIT 200T
1STEP COMP CELL PREP KIT 200T APEXBIO TECHNOLOGIES
Cayman Chemical DPP asy Buffer10X 1ea
For simple one-step purification of cysteinyl leukotrienes from most biological samples prior to analysis by EIA.
Medchemexpress LLC 1-Methylcytosine | 1122-47-0 | 125.13 | 1 ML
1-Methylcytosine is a pyrimidinone that functions as a nucleobase for hachimoji DNA, forming pairs with isoguanine. It exhibits weak antiproliferative activity against cervical cancer cells and is utilized in cervical cancer research.
- Serves as a nucleobase for hachimoji DNA
- Pairs with isoguanine
- Exhibits weak antiproliferative activity against cervical cancer cells
- Used in the research of cervical cancer
Medchemexpress LLC 5-Methylcytosine | 554-01-8 | 125.13 | 25 MG
5-Methylcytosine is a well-characterized DNA modification found in prokaryotes and eukaryotes. It forms symmetrical methylation on CpG dinucleotides in DNA, stabilizes tRNA/rRNA structure in RNA, and influences mRNA translation. This product can also be oxidized to produce 5hmC, 5fC, and 5caC.
- Used in epigenetics, developmental biology, and the study of diseases
- Forms symmetrical methylation on CpG dinucleotides in DNA
- Stabilizes tRNA/rRNA structure in RNA
- Influences mRNA translation
As One International Inc RealQ Plus 2x Master Mix for Probe without ROX 4000R, 4000Rxn
Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of SNP or quantitative gene transcripts. AS ONE offers the Real Q-PCR Master Mix, a single-tube 2X reagent ideal for most Quantitative PCR applications. The Real Q-PCR kit has been designed for optimal performance on most of the commonly used Real time PCR instruments. The Real Q-PCR kits includes the components necessary for performing DNA amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA. The Real Q-PCR master mix includes the HS DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The HS enzyme improves the PCR amplification reaction by decreasing background from non-specific amplification and increases amplification of desired products.
As One International Inc Red-Taq DNA Polymerase 1.1x Master Mix, 1.5 mM MgCl2 (final concentration), 10,000Rxn
AS ONE Taq DNA Polymerase with Red Dye Master Mix is a ready-to-use 1.1x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. An inert red dye and a stabilizer are also present to allow direct loading of the final products onto a gel for analysis. AS ONE Taq DNA Polymerase with Red Dye Mix offers several advantages. Set up time is significantly reduced. There is no need to buy and use separate loading dyes to load reaction products onto agarose gels for electrophoresis and subsequent visualization. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer and 10x Combination Buffer both Magnesium and detergent free, 10,000U
AS ONE HS DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Polymerase DNA Polymerase into the reaction. HS Taq includes Tween/Triton/magnesium free 10x Ammonium Buffer and 10x Combination Buffer
As One International Inc Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, Magnesium free and Tween free, 500U
AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. AS ONE Taq DNA Polymerase has both a 5 prime to 3 prime DNA polymerase and a 5 prime to 3 prime' exonuclease activity. The enzyme lacks a 3 prime to 5 prime exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A overhang, which makes the enzyme ideal for TA cloning. Included with Taq is Tween/magnesium free 10x Ammonium Buffer.
As One International Inc Taq DNA Polymerase 2x Master Mix, 1.5 mM MgCl2 (final concentration), 500Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 2.0X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 25 uL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Alkali Scientific Radiant™ cDNA Synthesis Kit, 1-Step, 5 Reactions, 1 x 0.2mL 5X cDNA Mix, 1 x 0.005mL 20X RTase
Radiant™ cDNA Synthesis Kit, 1-Step • Unbiased representation of 5’ and 3’ mRNA transcript ends• Sensitive detection of low copy number transcripts• High cDNA yields from as little as 4pg total RNA• Simple 2 tube systemSUPPLIED WITH a proprietary ratio of anchored oligo dT and random hexamers
