PCR Supplies
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 500 rxn (5 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 1000 rxn (10 x 1 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 200 rxn (2 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
Cayman Chemical 5-FAM-LL-37 (human) (trifluoroacetate salt), Purity: ≥90%, 10 mg
A fluorescently labeled form of LL-37; ex/em = 492/518 nm
Cayman Chemical Direct Red 80, CAS: 2610-10-8, 10 g
An anionic hexasulfonic acid dye; abs = 540 nm; has been used as a crosslinking dye for collagen or amyloids, as well as a dye for fabrics
Qiagen, Inc. QIAGEN Multiplex PCR Kit, for 100 x 50 µL multiplex PCR reactions
PCR Kit; Multiplex; For fast and easy Multiplex assay development without optimization; 100 x 50uL rxns
LGC GENOMICS LLC (Lucigen) FailSafe PCR 2X PreMix K, 2.5 mLTwelve 2X PreMixes, individual components of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post - optimization FailSafe PCR System.
The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time.We promise!
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase dNTPack
Taq DNA Polymerase is a highly processive 5-3 DNA polymerase that lacks 3-5 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase 5 U/mul
Taq DNA Polymerase is a highly processive 5-3 DNA polymerase that lacks 3-5 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM a strain lacking Taq I restriction endonuclease. The enzyme was cloned in E.coli.
Sigma Aldrich Fine Chemicals Biosciences fastStart Universal Probe
Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.fastStart(TM) Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX without modification or adjustments to the specific instrument or protocol. This ready-to-use 2x concentrated master mix contains all reagents (except primers probe and template) needed for running quantitative real-time DNA-detection assays including qPCR and two-step qRT-PCR in the hydrolysis probe detection format. fastStart(TM) Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT fast Real-Time PCR System or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler(R) Instruments.