PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript IV
Final Product Type	First-Strand cDNA
Quantity	Each
Concentration	5X
For Use With (Application)	Real Time PCR (qPCR)
Detection Method	qPCR
Format	Master Mix
Size (Final Product)	Up to 10 kb
Reaction Format	Master Mix
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Hot Start	Built-In Hot Start
Concentration	2X
For Use With (Application)	Hot-start PCR

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Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Pricing & Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
Polymerase	Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	Hot Start DNA Polymerase
Reaction Speed	Fast or Standard
Overhang	3'-A
Concentration	2X

Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Size (Final Product)	8 kb
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Universal Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	3'-A
Green Features	Less hazardous, less waste
Concentration	2X
For Use With (Application)	Hot-start PCR,Real Time PCR (qPCR)

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Content And Storage	SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type	Master Mix
Form	Frozen
Reaction Speed	Fast or Standard
Label or Dye	SYBR Green,FAM
Concentration	2X
For Use With (Application)	Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method	qPCR,2-step RT-qPCR,RT-qPCR
Detection Method	SYBR

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Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	ArrayScript™ UP
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	RNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Run Time	Standard
Product Line	RNA-to-C_T, SYBR

Invitrogen™ dNTP Mix (2.5 mM)

Invitrogen 2.5 mM dNTP (2'-deoxynucleoside 5'-triphosphate) Mix consists of a solution of all four nucleotides, dATP, dCTP, dGTP, and dTTP, each at a concentration of 2.5 mM.

Pricing & Availability

Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix

TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.

Pricing & Availability
Specifications

Content And Storage	Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Microbial Detection
PCR Method	qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Sensitivity	Down to 1 copy
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Frozen, Dry Ice
Content And Storage	Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Isolation Technology	Direct cell lysis
Kit Type	Direct Cell Lysis RT-qPCR
Green Features	REACH compliant, less waste
For Use With (Application)	Gene Expression
High-throughput Compatibility	Yes
PCR Method	2-step RT-qPCR
Detection Method	TaqMan
Target	cDNA
Format	Kit
Sample Type	Mammalian Cells
Purification Target	RNA
Starting Material	10-100,000 Cells

Thermo Scientific™ Phusion U Multiplex PCR Master Mixes

Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion U Hot Start DNA Polymerase
Fidelity (vs. Taq)	25X
Size (Final Product)	2.5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Multiplex PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X

Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Power SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Purity or Quality Grade	LD (Low DNA)
Reverse Transcriptase	M-MLV
Purification Time	5 min.
Green Features	Fewer resources used and less waste, Less hazardous
Concentration	20X
For Use With (Application)	RT-PCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cells
Reaction Speed	Standard
Label or Dye	SYBR Green
Hot Start	Built-In Hot Start
Product Line	Cells-to-CT, SYBR

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Pricing & Availability
Specifications

Fidelity (vs. Taq)	1X
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Overhang	3'-A
Concentration	5 U/μL
Starting Material	DNA
Exonuclease Activity	5' - 3'

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Pricing & Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Overhang	3'-A
Green Features	Sustainable packaging
Concentration	5 U/μL
For Use With (Application)	Hot-start PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Starting Material	DNA

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Pricing & Availability
Specifications

Shipping Condition	Room Temp or Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Overhang	3'-A
Green Features	Sustainable packaging
Concentration	2X
For Use With (Application)	Cloning,Hot-start PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq Gold 360 DNA Polymerase
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	PCR Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Starting Material	DNA

Applied Biosystems™ GeneAmp™ 10X PCR Buffer

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Pricing & Availability
Specifications

Chemical Name or Material	PCR Buffer
Concentration	10X
For Use With (Application)	Standard PCR

PCR Supplies

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Invitrogen™ Platinum™ Direct PCR Universal Master Mix Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix