The Zyppy™ Plasmid Miniprep Kit is the fastestand simplest method available to efficiently separate plasmid DNA from E. coli. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications. Simply add the uniquely formulated 7X Lysis Buffer directlyto your bacterial culture, neutralize, then purify using ourFast-Spin column technology (alternatively, the samples maybe processed by the classical centrifugation method).Additionally, the innovative colored buffers included in the kitpermit error-free visualization identification of completebacterial cell lysis and neutralization.