Real Time PCR Assays and Arrays
AURORA BIOLABS LLC Kras Q61H–cRAF Binding Assay Kit,384 rxn
KRAS is a small GTPase in the RAS protein family that regulates key cellular processes such as proliferation, differentiation, and survival through the RAS-RAF signaling pathway. Activation of KRAS occurs when GDP is exchanged for GTP, enabling interaction with downstream effectors including cRAF. Mutations in KRAS are frequently associated with human cancers, making the KRAS-cRAF interaction an important target for therapeutic development. The KRAS (Q61H)-cRAF Binding Assay Kit is a TR-FRET–based assay designed to measure the interaction between activated KRAS and cRAF. In this assay, Tag2-KRAS (Q61H) is loaded with GppNHp to mimic the active GTP-bound state, while Tag1-cRAF RBD binds activated KRAS. Binding generates a FRET signal detected by fluorescence emission at 665 nm and 620 nm. Inhibitors that disrupt KRAS-cRAF binding reduce the HTRF signal intensity.
AURORA BIOLABS LLC Kras Q61R Nucleotide Exchange Assay Kit,384 rxn
KRAS is a member of the RAS protein family, a group of small GTPases involved in cell signaling pathways that regulate cell growth, proliferation, differentiation, and survival. KRAS functions as a molecular switch by cycling between an inactive GDP-bound state and an active GTP-bound state. Activation of KRAS triggers downstream signaling pathways that promote normal cellular functions. Mutations in KRAS, such as the Q61R mutation, impair its GTPase activity and lock the protein in the active GTP-bound form, resulting in continuous signaling that drives uncontrolled cell proliferation and cancer development. KRAS mutations are frequently found in pancreatic, colorectal, and lung cancers. The KRAS Q61R nucleotide exchange assay is a TR-FRET–based assay designed to measure GTP binding activity and evaluate inhibitors targeting KRAS Q61R-mediated signaling pathways.
AURORA BIOLABS LLC Kras Q61R–cRAF Binding Assay Kit,100 rxn
KRAS is a small GTPase in the RAS protein family that regulates key cellular processes such as proliferation, differentiation, and survival through the RAS-RAF signaling pathway. Activation of KRAS occurs when GDP is exchanged for GTP, enabling interaction with downstream effectors including cRAF. Mutations in KRAS are frequently associated with human cancers, making the KRAS-cRAF interaction an important target for therapeutic development. The KRAS (Q61R)-cRAF Binding Assay Kit is a TR-FRET–based assay designed to measure the interaction between activated KRAS and cRAF. In this assay, Tag2-KRAS (Q61R) is loaded with GppNHp to mimic the active GTP-bound state, while Tag1-cRAF RBD binds activated KRAS. Binding generates a FRET signal detected by fluorescence emission at 665 nm and 620 nm. Inhibitors that disrupt KRAS-cRAF binding reduce the HTRF signal intensity.
LIFE TECHNOLOGIES TQMN SNP ASSAY NONHUMAN S 10
4351384 TQMN SNP ASSAY NONHUMAN S 10
AMID BIOSCIENCES Amid Biosciences, Human KRAS G12D Protein, Recombinant, AviTag, 0.1 mg
Human KRAS G12D is supplied as a recombinant AviTag protein in a 0.1 mg format for RAS pathway and oncology research. Applications include SPR, BLI, ELISA, Pull-down, AlphaScreen, MST, protein-protein interaction studies, drug discovery workflows. Technical specifications are included below.- Quantity - 0.1 mg- Gene - KRAS- Mutation / Variant - G12D- Organism - Human- Tag - N-terminal AviTag, N-terminal 8XHis- Expression System - E. coli- Purity - greater than 90% by Coomassie staining- UniProt ID - P01116-2- Amino Acid Range - 1-185- Buffer - 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol- Endotoxin Level - less than 1 EU/mg- Storage - -80 C- Biotinylated - NoThis product is for research use only.
Biotium CD71 / Transferrin Receptor (TFRC) (Extracellular Domain)(TFRC/2898R), 0.2mg/mL
Recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescenc
Biotium CD71 / Transferrin Receptor (TFRC) (Extracellular Domain)(TFRC/2898R), CF740 conjugate, 0.1mg/mL
Recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescenc