Real Time PCR Master Mixes
New England Biolabs, Inc. Quick-Load Taq 2X Master Mix – 500 reactions (50 µl vol)
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. Quick-Load Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl, tracking dyes and stabilizers. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR product onto agarose gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at approximately 4 kb and Orange G migrates at approximately 50 bp. The amount of tracking dyes included does not mask co-migrating DNA bands. Quick-Load Taq 2X Master Mix is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Apexbio Technology LLC 2HyPerFUsion High-Fidelity Master Mix (With dye), 1ml
Our product 2HyPerFUsion High-Fidelity Master Mix (With dye) offers superior performance for major PCR applications with high fidelity, powerful amplification ability and faster amplification speed. HyPerFUsion high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. The high fidelity makes hyPerFUsion high-fidelity DNA polymerase a superior choice for detection or other subsequent applications. HyPerFUsion high-fidelity DNA polymerase possesses one of the most accuracy with an error rate that is 50-fold lower compared to Taq DNA Polymerase and 6-fold lower than Pyrococcus Furiosus DNA Polymerase (Pfu). Other sizes are also available. Please inqury us for quote.
Biotium Fast Probe Master Mix (200 rxn)
Fast Probe Master Mix is formulated for conducting real-time PCR using oligo-based probes such as TaqMan or MGB probes. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq. Cheetah™ Taq is a chemically modified hotstart Taq which can be fully activated in 2 minutes, thus permitting fast PCR. We offer two formats of probe master mix, without ROX and with ROX.For sensitive, affordable qPCR reactions we now recommend our new and improved Forget-Me-Not™ Universal Probe PCR Master Mix.TaqMan is a registered trademark of Roche Diagnostics.
Biochain Institute Inc Eva QPCR SuperMix Kit, 400 rxn/PK
Components in these kits are prepared with pure chemicals according to our proprietary technology. BioChain's QPCR SuperMix is a 2x concentration of premix reagent including Hotstart DNA polymerase and specially formulated real time buffer designed for real-time PCR with intercalator format. Eva and SYBR Green QPCR Supermix is in double-stranded cDNA detection format, while Pro QPCR Supermix is in the format of fluorescent probe-based detection assays, such as TaqMan or molecular beacons.
Biochain Institute Inc QCell-Eva One-Step qRT-PCR SuperMix Kit, 200 rxn/PK
BioChain's QCell-Eva One-Step qRT-PCR SuperMix Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of gene expression directly from cells or RNA with intercalator format. QCell-Pro One-Step qRT-PCR SuperMix Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of gene expression directly from cells or RNA with probe based format.
New England Biolabs, Inc. LunaScript RT SuperMix Kit – 25 reactions
LunaScript RT SuperMix Kit features an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It includes the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix Kit contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.
Apexbio Technology LLC UNI. 2X PROBE QPCR MA. MIX 5ML
UNI. 2X PROBE QPCR MA. MIX 5ML APEXBIO TECHNOLOGIES
Apexbio Technology LLC UNI 2X FA GR QPCR MAS MIX 25ML
UNI 2X FA GR QPCR MAS MIX 25ML APEXBIO TECHNOLOGIES
New England Biolabs, Inc. AhdI – 5000 units
A restriction endonuclease that recognizes the sequence GACNN_N^NNGTC.
Thermo Scientific™ Luminaris Color Probe qPCR Master Mix, low ROX
Ready-to-use qPCR master mixes with color and premixed UDG optimized for probe detection using standard cycling protocols.
Thermo Scientific™ Luminaris Probe Low ROX qPCR Master Mixes
Ready-to-use qPCR master mixes with premixed UDG optimized for probe detection using standard cycling protocol. Master mixes available with or without blue color.