Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
ACROBiosystems E1A DNA 100tests
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy.In the context of biopharmaceutical production regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
ACROBiosystems CHO DNA 100tests
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy.In the context of biopharmaceutical production regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
ACROBiosystems HEK293T DNA 100tests
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy.In the context of biopharmaceutical production regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Enzo Life Sciences BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System (10Reactions)
The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis. This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 20 ng and up to 500 ng. Entire amplification reaction can be performed in a single tube and the biotin-labeled aRNA can be purified using either magnetic beads or purification columns. The BIOARRAY™ Low Input aRNA Amplification and Biotin Labeling System has been optimized for superior performance with reduced variability and improved reproducibility and data quality. The complete system is composed of reagents for cDNA synthesis and in vitro transcript labeling.
XpressBio XpressMyco qPCR Mycoplasma Detection Kit, 250 Reactions
The XpressMyco qPCR Mycoplasma Detection Kit is a real-time PCR (qPCR) assay for rapid, quantitative detection of mycoplasma contaminants in cell culture.
ACROBiosystems Plasmid resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Alkali Scientific Fast Optical qPCR Plates, WHITE, Low profile, Half-Skirted, 96 x 0.1ml Well, ABi Style A1notch, 5 bags of 10 plates, 50/Pk
Fast Optical qPCR Plates, WHITE, Low profile, HalfSkirted, 96 x 0.1mL Well, ABi Style A1notch, 5 bags of 10 plates, 50/pk
Alkali Scientific Radiant Green Flourescein qPCR Kit, 100 Reactions, (1 x 1mL)
Radiant Green Flourescein qPCR Kit, 100 Reactions, (1 x 1mL)
As One International Inc 50x Glass Blocking agent, 3 x 0.2 ml
One extra challenge using the LightCycler instrument is that the PCR reagents can form precipitates on the glass capillary surface as the Real time PCR progresses. To prevent this event AS ONE offers a special reagent for blocking the glass capillaries during the Quantitative PCR reaction. The Glass blocking agent is a 50x solution (1 ul per 50 ul PCR reaction).
As One International Inc RealQ Plus 2x Master Mix for Probe low ROX 4000R, 4000Rxn
Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of SNP or quantitative gene transcripts. AS ONE offers the Real Q-PCR Master Mix, a single-tube 2X reagent ideal for most Quantitative PCR applications. The Real Q-PCR kit has been designed for optimal performance on most of the commonly used Real time PCR instruments. The Real Q-PCR kits includes the components necessary for performing DNA amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA. The Real Q-PCR master mix includes the HS DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The HS enzyme improves the PCR amplification reaction by decreasing background from non-specific amplification and increases amplification of desired products.
As One International Inc RealQ Plus 2x Master Mix for Probe low ROX 400R, 400Rxn
Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of SNP or quantitative gene transcripts. AS ONE offers the Real Q-PCR Master Mix, a single-tube 2X reagent ideal for most Quantitative PCR applications. The Real Q-PCR kit has been designed for optimal performance on most of the commonly used Real time PCR instruments. The Real Q-PCR kits includes the components necessary for performing DNA amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA. The Real Q-PCR master mix includes the HS DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The HS enzyme improves the PCR amplification reaction by decreasing background from non-specific amplification and increases amplification of desired products.
Eagle Biosciences Inc Human Residual DNA Detection Kit (qPCR)
Human Residual DNA Detection Kit (qPCR)
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR Kit (No ROX) – 2500 reactions
In the Luna Probe One-Step RT-qPCR Kit (No ROX), Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed, ROX can be added.)