Cell Culture Growth Factors and Hormones
Cell Signaling Technology PathScanR RP Total BCKDH-E1-alpha Sandwich ELISA Kit 10 Kit
PathScanR RP Total BCKDH-E1-alpha Sandwich ELISA Kit 10 Kit
STEMCELL Technologies Human/Mouse Recombinant FGF-8B, ACF, Size: 1000 µg
Use fibroblast growth factor 8B (FGF-8B) to activate PI3K and MAPK pathways. FGF-8B has broad mitogenic and cell survival activities, and regulates gastrulation, epithelial-mesenchymal transition, and later on mesenchymal to epithelial differentiation during embryonic development (Klagsbrun. Prog Growth Factor Res, 1989). FGF-8B has mitogenic effects on somatic cells in the germinal epithelium and is expressed in adult mouse ovarian cells and tissues. This suggests that it regulates maturation of oocytes and seminiferous epithelium in testis (Valve et al. Biochem Biophys Res Commun, 1997). Human/mouse recombinant FGF-8B from STEMCELL comes lyophilized with ≥ 95% purity; specific activity EC50 ≤ 5 ng/mL; endotoxin levels verified ≤ 0.2 EU/μg protein. Alternative names: AIGF, Androgen-induced growth factor, FGF-8, FGF-8B, Fibroblast growth factor-8B, HBGF-8, Heparin-binding growth factor 8; HH6, KAL6. This product is animal component-free. Visit STEMCELL.com for more information.
STEMCELL Technologies Human Recombinant GM-CSF (CHO-expressed), Size: 1000 µg
Use granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages (Barreda et al. Dev Comp Immunol, 2004). Recombinant human GM-CSF also promotes the production of myeloid cells of the granulocytic and monocytic lineages in vivo. GM-CSF has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. In combination with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). Human recombinant GM-CSF (CHO-expressed) from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 0.2 ng/mL; endotoxin levels verified ≤ 0.2 EU/μg protein. Alternative names: Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha. Visit STEMCELL.com for more information.
STEMCELL Technologies Human Recombinant FGF-16, Size: 50 µg
Fibroblast growth factor 16 (FGF-16) is a heparin-binding member of the FGF family. FGFs possess broad mitogenic and cell survival activities and are expressed during embryonic development. FGFs act primarily on cells of mesodermal and neuroectodermal origin to regulate diverse physiological functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, and tissue repair (Goldfarb; Green et al.). In vitro, FGF-16 has been shown to promote the proliferation of brown adipocytes and in rat embryos it is predominantly expressed in these cells. FGF-16 has also been shown to play a critical role in embryonic heart development and is thought to play a cardioprotective role after birth (Hotta et al.; Lu et al.; Wang et al.).
STEMCELL Technologies Human Recombinant HBEGF, ACF, Size: 1000 µg
Heparin-binding epidermal growth factor (EGF)-like growth factor (HBEGF) is a member of the EGF family (Nishi & Klagsbrun). HBEGF promotes blastocyst adhesion to the uterine wall (Iwamoto & Mekada). It also plays a role in smooth muscle cell hyperplasia and brain injury (Nishi & Klagsburn). HBEGF produced by CD4+ T cells promotes wound healing by stimulating migration and proliferation of keratinocytes, fibroblasts, and smooth muscle cells (Blotnick et al.). It binds to EGFR, ErbB4, ErbB2, and ErbB3, activating the PI3K/AKT signaling cascade (Iwamoto & Mekada). HBEGF is produced in a variety of cells, where it contributes to physiological and pathological processes. HBEGF is overexpressed in ovarian, breast, gastric, colorectal, pancreatic, and endometrial cancers, which likely contributes to pathogenesis (Miyata et al.). This product is animal component-free.
STEMCELL Technologies Human Recombinant Persephin, ACF, Size: 1000 µg
Persephin is a neurotrophic factor that belongs to the glial cell line-derived neurotrophic factor (GDNF) family. Persephin shares a large degree of structural similarity to GDNF, artemin, and neurturin, and has overall neuroprotective activity. Persephin signals through GRFα4 (glycosylphosphatidylinositol (GPI)-linked GDNF receptor family member) which signals through the receptor tyrosine kinase RET. Unlike GDNF and neurturin, persephin only promotes the growth and survival of central dopaminergic and motor neurons, but not peripheral neurons (Milbrandt et al.). In vitro persephin only promotes survival of neurons that co-express GPI-linked GRFα4 and RET (Enokido et al.; Lindahl et al.). Mice lacking persephin showed increased cell death after cerebral ischemia, however administration of persephin before ischemia dramatically reduced neuronal cell death (Tomac et al.). This product is animal component-free.
STEMCELL Technologies Human Recombinant VEGF-D, Size: 50 µg
Vascular endothelial growth factor D (VEGF-D) is a member of the VEGF/platelet-derived growth factor (PDGF) family of proteins. VEGF-D is a potent angiogenic factor and promotes lymphangiogenesis, endothelial cell growth and survival, and can affect blood vessel permeability. VEGF-D is expressed in the lung, heart, small intestine, fetal lung, and at lower levels in the pancreas, colon, and skeletal muscle (Otrock et al.; Roy et al.; Stacker et al.; Yamada et al.). VEGF-D is a ligand for VEGF receptors 2 (Flk1) and 3 (Flt4) (Baldwin et al.). VEFGR-3 is highly expressed in lymphatic endothelial cells and is essential for their growth and differentiation (Otrock et al.; Roy et al.). Binding of VEGF-D to neuropilins contributes to VEGFR-3 signaling during lymphangiogenesis, whereas binding to integrin α9β1 promotes endothelial cell adhesion and migration (Roy et al.; Otrock et al.). During embryogenesis, VEGF-D also plays a role in the formation of the venous and lymphatic systems.
STEMCELL Technologies Human Recombinant PDGF-DD, Size: 50 µg
The platelet-derived growth factor (PDGF) family has five heparin-binding members that assemble into four homodimers (PDGF-AA, PDGF-BB, PDGF-CC, and PDGF-DD) and one heterodimer (PDGF-AB; Fretto et al.; Li & Eriksson). PDGF signals through the receptor tyrosine kinases PDGFRα and PDGFRβ. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). PDGF-DD promotes growth and survival of renal artery smooth muscle cells and lens epithelial cells, and can act as a macrophage chemoattractant (Changsirikulchai et al.; Lokker et al.; Ray et al.; Uutela et al.).
STEMCELL Technologies Human Recombinant Persephin, Size: 100 µg
Persephin is a neurotrophic factor that belongs to the glial cell line-derived neurotrophic factor (GDNF) family. Persephin shares a large degree of structural similarity to GDNF, artemin, and neurturin, and has overall neuroprotective activity. Persephin signals through GRFα4 (glycosylphosphatidylinositol (GPI)-linked GDNF receptor family member) which signals through the receptor tyrosine kinase RET. Unlike GDNF and neurturin, persephin only promotes the growth and survival of central dopaminergic and motor neurons, but not peripheral neurons (Milbrandt et al.). In vitro, persephin only promotes survival of neurons that co-express GPI-linked GRFα4 and RET (Enokido et al.; Lindahl et al.). Mice lacking persephin showed increased cell death after cerebral ischemia, however administration of persephin before ischemia dramatically reduced neuronal cell death (Tomac et al.).
STEMCELL Technologies Human Recombinant VEGF-121, Size: 1000 µg
Vascular endothelial growth factor (VEGF-121) is a naturally-occurring VEGF-A splice variant involved in embryonic vasculogenesis and angiogenesis. VEGF binds to VEGFR-1 and VEGFR-2, and activates Raf/MEK/ERK and PI3K/AKT pathways. VEGF-121 is released as a freely diffusible protein by a variety of normal and transformed cells. It plays an important role in neurogenesis both in vitro and in vivo (Storkebaum et al.). It has neurotrophic effects on neurons of the central nervous system and promotes growth and survival of dopaminergic neurons and astrocytes. VEGF also promotes growth and survival of vascular endothelial cells, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells (Ferrara et al.).
STEMCELL Technologies Mouse Recombinant M-CSF, Size: 1000 µg
Use macrophage colony-stimulating factor (M-CSF) to regulate the proliferation and differentiation of myeloid hematopoietic progenitor cells to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a homodimeric glycoprotein growth factor that is crucial to the development of tissue-resident macrophages in most tissues (Ginhoux & Jung Nat Rev Immunol, 2014) and regulates inflammatory responses together with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al. Immunity, 2014). M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) (Hamilton JA J Leukoc Biol, 1997). Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam & Ghosh Tumour Biol, 2014). Mouse recombinant M-CSF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 10 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: Colony stimulating factor 1, CSF-1.
STEMCELL Technologies Human Recombinant GM-CSF, ACF, Size: 1000 µg
Use granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages (Barreda et al. Dev Comp Immunol, 2004). Recombinant human GM-CSF also promotes the production of myeloid cells of the granulocytic and monocytic lineages in vivo. GM-CSF has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. In combination with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). Human recombinant GM-CSF from STEMCELL is animal component-free and comes lyophilized with ≥ 95% purity; EC50 ≤ 200 pg/mL; endotoxin levels verified ≤ 1.0 EU/μg protein. Alternative names: Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha. Visit STEMCELL.com for more information.
Cayman Chemical Humn MInralocortIcoId Recep
MR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.[INDIGO Catalog Nos. IB00501-32, IB00501, IB00502]
Cayman Chemical Humn VItamIn D RECPTR Repo 384
VDR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.[INDIGO Catalog Nos. IB00701-32, IB00701, IB00702]
Medchemexpress LLC LGP2 protein, human (HEK293, FLAG) | >90.0% | 10UG
Recombinant human LGP2 protein expressed in HEK293 cells with a C-terminal FLAG tag. The protein regulates RIG-I and MDA5-mediated antiviral signaling and is supplied as a purified, buffered solution intended for research applications. Provided in small microgram quantities, the product includes storage and handling recommendations to preserve activity and integrity.
- Expressed in HEK293 cells with C-terminal FLAG tag.
- Purity greater than 90% by reducing SDS-PAGE.
- Formulated in 50 mM HEPES, pH 7.5; 200 mM NaCl; 20% glycerol; 1 mM DTT.
- Available in 10 μg, 50 μg, and 100 μg sizes.
- Store at -80°C; stable at -20°C for short-term; avoid repeated freeze-thaw cycles.
- Molecular weight approximately 77.6 kDa (coverage M1-D678).