The System is our Complete All-In-One kit optimized to elevate your experiments by providing everything needed to plate, culture, passage, and freeze cells in a convenient and consistent manner. The System includes 2x500mL Classic Complete Media (4Z0-500) formulated at 10% serum, 2x5mL CultureBoost animal-derived growth supplement to complete medium, 1x100mL Attachment Factor for healthy cell attachment, 1x50mL Cell Freezing Medium, and 1 set 100mL Passage Reagent Group (PRG) for cell release. PRG contains 3 parts: 1x100mL PRG-1 (EDTA-dPBS Solution), 1x100mL PRG-2 (Trypsin/EDTA-dPBS Solution) & 1x100mL PRG-3 (Trypsin Inhibitor-dPBS Solution). Large quantities? Contact your Fisher Scientific sales representative to discuss your bulk order needs. Research on a tight deadline? Available for next day delivery after the purchase order is received. Maximize your results! Try our Primary Human Cells which are ideal for use with our media and reagents.