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For researchers, scientists, and technical professionals: Your one-stop shop for the complete range of laboratory, production, and safety products and services.
A broad variety of liquid solutions, reagents, and dry compounds designed to support the growth, proliferation, and cryopreservation of cells; various formulations and volumes available for an assortment of cell types.
Minimum Essential Medium (MEM) developed by Harry Eagle is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagles Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.MEM which incorporates these modifications includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks or Earles salts has broadened the usefulness of this medium.
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This DMEM f-12 Ham medium differs from the original formula because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
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