Cell Culture Media
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Promocell GmbH PromoCell Fibroblast Basal Medium 500 ml
PromoCell Fibroblast Growth Medium is optimized for the cultivation of neonatal and juvenile fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vialsWhile the supplements of the Fibroblast Growth Medium are serum-free, the Basal Medium is manufactured with 0.5% serum.| Media supplement | Final supplement concentration|| Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Supplement Mix / Myocyte Growth Medium
The PromoCell Myocyte Growth Medium is a low serum (5% V/V) medium supplemented with well chosen hormones and growth factors. It was developed for the optimal expansion and maintenance of primary cardiac myocytes in culture.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine and primary rat cardiac myocytes.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 0.5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 2 ng / ml || Insulin (recombinant human) | 5 µg / ml |
Promocell GmbH PromoCell Supplement Mix / Airway Epithelial Cell Growth Medium
PromoCell Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, bat and rat airway epithelial cells.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml |
Promocell GmbH PromoCell Fibroblast Basal Medium 3 500 ml
PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF).Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine cardiac fibroblasts.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.1 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Chondrocyte Basal Medium (prf) 500 ml, phenol red free
PromoCell Chondrocyte Growth Medium has been developed for the in vitro cultivation of human chondrocytes. The fetal calf serum in the medium is strictly pretested for optimal performance.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for primary porcine and rat chondrocytes.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.1 ml / ml |
Promocell GmbH PromoCell Endothelial Cell Basal Medium MV 2 500 ml
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell Endothelial Cell Basal Medium MV 2 (prf) 500 ml, phenol red-free
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell Supplement Mix / Endothelial Cell Growth Medium MV 2
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell Adipocyte Nutrition Medium 500 ml, ready-to-use, low serum, with C-39438 attached to the flask
PromoCell Adipocyte Nutrition Medium is a low-serum (3% V/V) medium developed for the in vitro cultivation of mature adipocytes.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine preadipocytes/adipocytes.Components:* Nutrition Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Nutrition Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.03 ml / ml || d-Biotin | 8 μg / ml || Insulin (recombinant human) | 0.5 μg / ml || Dexamethasone | 400 ng / ml |
Promocell GmbH PromoCell DC Base Medium XF 250 ml, with C-39854 attached to flask
The PromoCell Dendritic Cell (DC) Generation Media has been developed for the easy and efficient generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes. Both the DC Base Medium XF and DC Generation Medium XF (Ready-to-use) feature a serum-free and xeno-free formulation for use with freshly isolated cellsThe DC Base Medium XF consist of a bottle of Basal Medium and one vial of SupplementMix. However, it does not include cytokines and must therefore be supplemented according to the user's individual needs.The DC Generation Medium XF (Ready-to-use) consists of a bottle of Basal Medium, one vial of SupplementMix, and CytokineMix containing all necessary differentiation factors.
Promocell GmbH PromoCell Keratinocyte Basal Medium 2 500 ml, calcium-free
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.125 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.33 μg / ml || Epinephrine | 0.39 μg / ml || Transferrin (recombinant human) | 10 μg / ml || CaCl2 | 0.06 mM |
Biotang Inc TBS/TWEEN-20 (10X) 500 ML, IBB-580, 1% Tween-20 in 250 mM Tris-HCl, 27 mM KCl and 1.37 M NaCl, pH 7.4 +/- 0.2. Prepared in 18.2 megohm water.and autoclaved at 15 psi for 30 min on liquid cycle.
TBS/TWEEN-20 (10X) 500 ML, IBB-580, 1% Tween-20 in 250 mM Tris-HCl, 27 mM KCl and 1.37 M NaCl, pH 7.4 +/- 0.2. Prepared in 18.2 megohm water.and autoclaved at 15 psi for 30 min on liquid cycle.
STEMCELL Technologies CryoStor CS2, Size: 100 mL
Maximize post-thaw cell recovery and viability following cryopreservation at low temperatures (-80°C to -196°C) with ready-to-use CryoStor CS2. Serum-free, animal component-free, and cGMP-manufactured, this defined medium provides a safe, protective environment for cells and tissues during freezing and thawing processes and storage. CryoStor CS2 is formulated with USP-grade components and contains 2% DMSO.
