Additional Selective and Differential Cell Culture Media
NEOGEN EC Medium, Modified with Novobiocin, 5 kg
EC Medium, Modified with Novobiocin is used for the selective enrichment of Escherichia coli O157:H7 in a laboratory setting. EC Medium, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria and fecal streptococci were inhibited by the bile salts.EC Medium, Modified with the addition of Novobiocin was first described by Okrend and Rose. Okrend and Rose modified EC Medium by reducing the Bile Salts Mixture concentration to 1.12% and adding 20 mg/L of sodium novobiocin.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-modified-with-novobiocin
Biolegend APC/Cyanine7 anti-human CD272
APC/Cyanine7 anti-human CD272 (BTLA) [MIH26]; Isotype: Mouse IgG2a, κ; Reactivity: Human; Apps: FC; Size: 25 tests
NEOGEN LB Broth, Lennox, 500 g
LB Broth, Lennox is used in molecular genetic studies in a laboratory setting. LB Broth, Lennox is not intended for use in the diagnosis of disease or other conditions in humans.LB Broth formula is based on L Broth described by Lennox for growth and maintenance of E. coli strains used in molecular microbiology procedures. LB Broth is nutritionally rich, formulated for the isolation of pure recombinant strains. E. coli is grown to late log phase in LB Broth. Some plasmid vectors may replicate to high copy numbers without selective amplification. Some vectors may require selective amplification to reach high copy numbers. Chloramphenicol can be added to inhibit host synthesis and, as a result, prevent replication of the bacterial chromosome.For more information visit https://www.neogen.com/solutions/Microbiology/lb-broth-lennox
NEOGEN Modified Giolitti & Cantoni Broth, 500 g
Modified Giolitti and Cantoni Broth (ISO) is used for the detection and enumeration of coagulase- positive staphylococci from food and animal feeding stuffs using the Most Probable Number (MPN) technique according to ISO 6888-3:2003. This medium is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Giolitti and Cantoni as a medium for the enrichment of staphylococci from foodstuffs, Mossel later applied the medium to use with samples from dried milk and infant food. Optimized for use in samples where staphylococci may be stressed and/or in low numbers, growth of the target organisms is promoted by sodium pyruvate, Glycine and the high concentration of mannitol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-giolitti-and-cantoni-broth
NEOGEN Terrific Broth, Modified, 10 kg
Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans. Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli and is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.For more information visit https://www.neogen.com/solutions/Microbiology/terrific-broth-modified
NEOGEN Buffered Sodium Chloride - Peptone Broth pH 7.0, 10 kg
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans.Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-sodium-chloride-peptone-broth-ph-7
NEOGEN Eosin Methylene Blue (EMB) Agar Levine, 500 g
Eosin Methylene Blue Agar Levine is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.This medium was introduced in 1916 by Holt-Harris and Teague to differentiate Escherichia spp. and Aerobacter spp. It was modified by Levine in 1918 who removed sucrose from the formula and increased the lactose content. The distinctive metallic sheen produced by E. coli on this medium is due to acid production resulting in an amide bonding between the eosin and methylene blue, other coliforms do not produce enough acid to cause this reaction. Eosin inhibits most Gram-positive organisms. The prepared medium is sensitive to light.For more information visit https://www.neogen.com/solutions/Microbiology/eosin-methylene-blue-agar-levine
NEOGEN MRS Agar, 500 g
MRS Agar is a medium for the enumeration of mesophilic lactic acid bacteria according to ISO 15214:1998, and is not intended for use in the diagnosis of disease or other conditions in humans. MRS Agar was originally developed in 1960 by de Man, Rogosa & Sharpe for the cultivation and enumeration of Lactobacillus spp. from various sources and is intended as a substitute for Tomato Juice Agar. This original formulation has been adapted and adjusted to pH 5.7 according to ISO 15214:1998. Nutrition is provided by enzymatic digest of casein, glucose, meat & yeast extracts whilst polyoxyethylenesorbitan monooleate, magnesium and manganese sulphates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli whilst inhibiting a number of other organism groups.For more information visit https://www.neogen.com/solutions/Microbiology/mrs-agar
NEOGEN Dermatophyte Test Medium (DTM), 500 g
Dermatophyte Test Medium is used for the selective isolation of dermatophytic fungi and is not intended for use in the diagnosis of disease or other conditions in humans.In 1969, Taplin et al. developed this medium for the isolation and recognition of dermatophytic fungi, the causative agent of ringworm from hair, nails, and skin. Dermatophyte Test Medium is preferred for isolation and early recognition of Microsporum, Trichophyton, and Epidermophyton genera because of a distinct color change in the medium. Rapidly-growing species may produce a complete color change in the medium in 3 days. The slower-growing species will change the indicator in longer time periods. Other organisms may grow but can be recognized as non-dermatophytes by lack of a color change.For more information visit https://www.neogen.com/solutions/Microbiology/dermatophyte-test-medium-dtm
Promocell GmbH PromoCell Mammary Epithelial Cell Growth Medium 500 ml, ready-to-use, serum-free, with C-39115 attached to the flask
The PromoCell Mammary Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the mammary gland.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for rat and murine mammary epithelial cells as well as mamma carcinoma cell lines.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml |
Sigma Aldrich Fine Chemicals Biosciences fibroblast Growth Medium 2
fibroblast Growth Medium 2 (Ready-to-use)
Promocell GmbH PromoCell Renal Epithelial Cell Growth Medium 2 KIT 500 ml, Contains: C-26230, C-39605
PromoCell Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for porcine and mouse renal epithelial cells.| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 36 ng / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 4 pg / ml || Transferrin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Preadipocyte Differentiation Medium KIT 500 ml, Contains: C-27411, C-39437
The Preadipocyte Differentiation Medium is a defined and animal-component free medium, which induces the differentiation of preadipocytes into mature adipocytes. The mature adipocytes can be cultivated using the Adipocyte Nutrition Medium.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine preadipocytes/adipocytes.Components:* Differentiation Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Differentiation Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration || d-Biotin | 8 μg / ml || Insulin (recombinant human) | 0.5 μg / ml || Dexamethasone | 400 ng / ml || IBMX | 44 μg / ml || L-Thyroxine | 9 ng / ml || Ciglitazone | 3 μg / ml |
Promocell GmbH PromoCell Preadipocyte Basal Medium 500 ml
PromoCell Preadipocyte Growth Medium is a low-serum (5% V/V) medium developed for the undifferentiated expansion of human preadipocytes.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine preadipocytes/adipocytes.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.05 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Hydrocortisone | 1 μg / ml || Heparin | 90 μg / ml |
Promocell GmbH PromoCell Supplement Mix / Keratinocyte Growth Medium 2
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.125 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.33 μg / ml || Epinephrine | 0.39 μg / ml || Transferrin (recombinant human) | 10 μg / ml || CaCl2 | 0.06 mM |