Additional Selective and Differential Cell Culture Media
NEOGEN Violet Red Bile Agar (VRBA) (ISO), 5 kg
A medium for the enumeration of coliform organisms in food and dairy products according to ISO 4832:2006 and is not intended for use in the diagnosis of disease or other conditions in humans.The selectivity of the medium is due to the presence of bile salts and crystal violet. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same color. Non-lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42-44°C.Formula:Yeast Extract - 3.0 g/LEnzymatic Digest of Animal Tissue - 7.0 g/LBile Salts - 1.5 g/LLactose - 10.0 g/LNeutral Red - 0.03 g/LCrystal Violet - 0.002 g/LAgar- 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-lactose-agar-iso
NEOGEN EC Broth, 500 g
EC Broth is used for the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C. EC Broth is not intended for use in the diagnosis of disease or other conditions in humans.EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC.EC Broth is made selective for coliforms by the inclusion of Bile Salts in the dehydrated medium. The selective nature of this medium ensures that the growth of non-coliform bacteria is minimized. The medium is buffered by the addition of potassium phosphates and osmotically balanced by sodium chloride. The medium is used at 37°C for coliform organisms and 44.5°C is recommended for the isolation E. coli.For more information visit https://www.neogen.com/solutions/Microbiology/ec-broth
NEOGEN Buffered Peptone Water (BPW), 10 kg
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water
NEOGEN LB Broth, Lennox, 500 g
LB Broth, Lennox is used in molecular genetic studies in a laboratory setting. LB Broth, Lennox is not intended for use in the diagnosis of disease or other conditions in humans.LB Broth formula is based on L Broth described by Lennox for growth and maintenance of E. coli strains used in molecular microbiology procedures. LB Broth is nutritionally rich, formulated for the isolation of pure recombinant strains. E. coli is grown to late log phase in LB Broth. Some plasmid vectors may replicate to high copy numbers without selective amplification. Some vectors may require selective amplification to reach high copy numbers. Chloramphenicol can be added to inhibit host synthesis and, as a result, prevent replication of the bacterial chromosome.For more information visit https://www.neogen.com/solutions/Microbiology/lb-broth-lennox
NEOGEN Meat Peptone, 5 kg
Meat Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.Meat Peptone is a highly nutritious enzymatic digest of meat and may be used as an ingredient in culture media.For more information visit https://www.neogen.com/solutions/Microbiology/meat-peptone
NEOGEN Oxford Listeria Agar, 10 kg
Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water.For more information visit https://www.neogen.com/solutions/Microbiology/oxford-listeria-agar
NEOGEN PALCAM Broth, 10 kg
PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.For more information visit https://www.neogen.com/solutions/Microbiology/palcam-broth
NEOGEN Yeast Extract Dextrose Chloramphenicol Agar, 500 g
Yeast Extract Dextrose Chloramphenicol Agar is a selective medium for the enumeration of yeasts and molds in milk and other dairy products, and is not intended for use in the diagnosis of disease or other conditions in humans. The medium is said to have superior storage properties to O.G.Y.E. and also has the advantage of incorporating an autoclavable supplement.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract-dextrose-chloramphenicol-agar
NEOGEN Violet Red Bile Glucose Agar (VRBGA), 500 g
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods. Violet Red Bile Glucose Agar conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of bile-tolerant Gram-negative bacteria from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Yeast extract provides vitamins and gelatin serves as source of carbon and nitrogen. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. The formulation is a modification of Violet Red Bile Agar by Mossel which substitutes lactose for glucose.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar
NEOGEN Hektoen Enteric (HE) Agar, 500 g
Hektoen Enteric Agar is used for the isolation and differentiation of enteric pathogens. Hektoen Enteric Agar is not intended for use in the diagnosis of disease or other conditions in humans. Hektoen Enteric Agar was developed in 1967 by King and Metzger. Compared to other enteric differentiating media commonly used, Hektoen Enteric Agar increased the isolation rate of Salmonella spp. and Shigella spp. This was accomplished by increasing the carbohydrate and peptone content of the medium in order to counteract the inhibitory effects of bile salts and indicators. King and Metzger formulated a medium that slightly inhibited growth of Salmonella and Shigella, while inhibiting Gram-positive microorganisms. Enzymatic Digest of Animal Tissue provides nitrogen, carbon, and amino acids required for organism growth.For more information visit https://www.neogen.com/solutions/Microbiology/hektoen-enteric-agar
NEOGEN Yeast Extract, 10 kg
Yeast Extract is an autolysate of yeast cells used in preparing microbiological culture media in a laboratory setting. Yeast Extract is not intended for use in the diagnosis of disease or other conditions in humans.Yeast Extract is the water-soluble portion of autolyzed yeast. The autolysis is carefully controlled to preserve naturally occurring B-complex vitamins. Yeast Extract is prepared and standardized for bacteriological use and cell cultures and is an excellent stimulator of bacterial growth. Yeast Extract is generally employed in the concentration of 0.3% - 0.5%. Yeast Extract is typically prepared by growing baker’s yeast, Saccharomyces spp., in a carbohydrate-rich plant medium. The yeast is harvested, washed, and resuspended in water, where it undergoes autolysis, i.e., self-digestion using the yeast’s enzymes.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract
NEOGEN Middlebrook 7H11 Agar, 5 kg
Middlebrook 7H11 Agar is used with glycerol and OADC Enrichment for the cultivation of Mycobacterium spp. in a laboratory setting. Middlebrook 7H11 Agar is not intended for use in the diagnosis of disease or other conditions in humans.There are two types of solid culture media for the primary isolation of mycobacteria, coagulated egg as a base (Lowenstein formulations) and an agar base (Middlebrook formulations). The use of agar-based media for primary isolation of mycobacteria have the following significant advantages:Agar-based media do not usually liquefy in the presence of contaminating proteolytic organisms.Agar-based media retain exact concentrations of added drugs because the medium is solidified with agar rather than by inspissation of the egg. There is less drug inactivation when egg ingredients are absent.For more information visit https://www.neogen.com/solutions/Microbiology/middlebrook-7h11-agar
NEOGEN DRBC Agar (BAM), 500 g
DRBC Agar (BAM) is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. DRBC Agar is not intended for use in the diagnosis of disease or other conditions in humans.DRBC Agar (BAM) is based on Dichloran Rose Bengal Chlortetracycline (DRBC) Agar formula described by King, Hocking, and Pitt. DRBC Agar conforms with APHA guidelines for the mycological examination of foods containing chloramphenicol rather than chlortetracycline as proposed by King, Hocking, and Pitt. DRBC Agar is a selective medium, supporting good growth of yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-bam
NEOGEN Lactobacilli MRS Broth, 500 g
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-broth
NEOGEN Harlequin Cronobacter Isolation Agar, 500 g
Chromogenic Cronobacter Isolation Agar (CCI) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Tryptic digest of casein and yeast extract provide a source of nitrogen and essential vitamins. The high content of sodium chloride and the presence of sodium desoxycholate produce a selective and specific medium.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-cronobacter-isolation-agar