MethoCult™ H4531 is methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, peripheral blood, mobilized peripheral blood, and cord blood samples. This formulation contains agar leukocyte conditioned medium (agar-LCM) as a source of colony-stimulating factors. MethoCult™ H4531 supports the growth of granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M) and EPO-independent erythroid progenitor cells. It is suitable for detection of EPO-independent erythroid progenitor cells.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/technical-resources/educational-materials/tech-tips/frequently-asked-questions-cfu-assay.html) on performing the CFU assay and explore its utility as part of the cell therapy workflow (https://www.stemcell.com/technical-resources/educational-materials/tech-tips/cfu-cell-therapy-questions.html#section-applications).