Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay PAC1-long Receptor Frozen Cells, 2 Vials of Cells. (HTS114RTA)
The Ready-to-Assay PAC1-long Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADCYAP1R1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADCYAP1R1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen BB1 Bombesine/Neuromedin B Receptor Stable Cell Line, 2 vials. (HTS123C)
The ChemiScreen BB1 Bombesin/Neuromedin B Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen FPRL1 N-Formylpeptide Receptor Stable Cell Line, 2 vials. (HTS056C)
The ChemiScreen FPRL1 N-Formylpeptide Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. N-formyl peptides) based activation of FPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FPR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of FPR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Streptococcus pneumoniae
Streptococcus pneumoniae has applications in respiratory disease research. This bacterium is incubated in a 5% CO2 atmosphere to enhance growth.
ATCC AFT024; Embryonic liver; Mouse (Mus musculus)
AFT024 is a fibroblast cell that was isolated from the stroma of a mouse. This cell has applications in stem cell research. SCRC-1007 can serve as a support layer for stem cells at 37°C; however, to ensure that all AFT024 cells are mitotically inactive, irradiation or Mitomycin C treatment is recommended.
ATCC Aspergillus brasiliensis; WLRI 034(120)
Lyophilized fungal spores and mycelium residue in a vial
ATCC Enterococcus faecalis
Enterococcus faecalis strain CN 478 is a quality control strain that can be used in susceptibility testing and the evaluation of Mueller-Hinton Agar.
ATCC Metarhizium frigidum
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Pseudomonas sp.; Strain: RH 926
Pseudomonas sp. strain RH 926 is a whole-genome sequenced bacterium that is propagated aerobically.
Biochain Institute Inc Frozen Tissue Section - Human Tumor: Stomach, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biochain Institute Inc Frozen Tissue Section - Alzheimer's Disease: Brain: Pons, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx U2OS GRM1 Gq Cell Line, 2 vials. (95-1021C3)
The GRM1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. L-glutamate) based activation of GRM1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRM1, a naturally Gq coupled GPCR. Upon activation of GRM1, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx CHO-K1 FFAR1 (GPR40) Gq Cell Line, 2 vials. (95-1005C2)
The FFAR1 (GPR40) Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. FFAs) based activation of FFAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR1, a naturally Gq coupled GPCR. Upon activation of FFAR1, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.