Cell Lines and Cell Cultures
ATCC Candida albicans; genomic DNA
Purified genomic DNA (deoxyribonucleic acid), 5 micrograms, dried. Isolated from Candida albicans ATCC 14053 (strain NIH 3172).
ATCC Staphylococcus; haemolyticus; SM131 genomic DNA
Genomic DNA isolated from Staphylococcus haemolyticus strain SM 131. This bacterial strain is also available as ATCC Catalog No.: 29970™.
ATCC Bacillus cereus; Strain FDA 5; genomic DNA
Genomic DNA isolated from Bacillus paranthracis strain FDA 5 (ATCC 10702). This whole-genome sequenced product can be used in PCR and other molecular biology applications.
ATCC Escherichia coli; Quantitative DNA
Quantitative Genomic DNA from Escherichia coli strain 2003-3014 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Eurofins Discoverx Ready-to-Assay M4 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS117RTA)
The Ready-to-Assay M4 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM4 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM4, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen α2B Adrenergic Receptor Membrane Preparation, 1 vial. (HTS157M)
The ChemiScreen α2B Adrenergic Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRA2B surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen C3aR Anaphylotoxin Receptor Membrane Preparation, 1 vial. (HTS016M)
The ChemiScreen C3aR Anaphylotoxin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of C3AR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC Mycoplasma synoviae
Mycoplasma synoviae strain WVU 1853 [NCTC 10124] is a whole-genome sequenced type strain that was isolated from the hock joint of a chicken. This bacterial culture has applications in agricultural research, media testing, quality control, and pharmaceutical testing.
ATCC Quantitative Synthetic; Human herpesvirus; 6A DNA
Quantitative synthetic Human herpesvirus 6A DNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. This preparation includes fragments from U31 (large tegument protein), U38 (DNA polymerase), U57 (major capsid protein), U65/U66, U67, U90 (IE1 / IE-A protein), and U94 regions.
Eurofins Discoverx ChemiScreen CXCR7 Chemokine Receptor Membrane Preparation, 1 vial. (HTS138M)
The ChemiScreen CXCR7 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CXCR7 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen ChemR23 Chemoattractant Receptor Stable Cell Line, 2 vials. (HTS071C)
The ChemiScreen ChemR23 Chemoattractant Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CMKLR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CMKLR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay 5-HT2A Serotonin Receptor Frozen Cells, 2 Vials of Cells. (HTS082RTA)
The Ready-to-Assay 5-HT2A Serotonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. serotonin/5-HT) based activation of HTR2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2A alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2A, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen EP3 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS092C)
The ChemiScreen EP3 Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Vero, Select Kidney; Monkey (Chlorocebus aethiops) Grivet
The Vero Select cell line was derived from Vero, CCL-81. The cell line can be used in a variety of applications, including the detection of verotoxins, detection of virus in ground beef, efficacy testing, the study of malaria, media testing, vaccine development, protein expression, and mycoplasma testing. The Vero cell line is also a suitable transfection host.