Quantitative synthetic RNA for Zika virus can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. This preparation includes fragments from the Membrane glycoprotein precursor M, Envelope, NS1, NS2B, NS3, NS4B, and NS5 regions.