Quantitative synthetic DNA for Human gammaherpesvirus 4 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load and for other molecular biology applications. This preparation includes LMP2, BNRF-1, EBER-1, BAMH1W (1 copy, virus genome contains 7), EBNA-2, BHRF-1, EBNA-1 Region (BERF3, BKRF1), BXLF-1, BALF-5, and LMP-1.