General Cell Lines and Cell Cultures
ATCC Mycoplasma orale; Strain CH 19299; genomic DNA
Genomic DNA from Mycoplasma orale strain CH 19299 (ATCC 23714). This product can be used in PCR and other molecular biology applications.
ATCC Klebsiella pneumoniae; subsp. pneumoniae; Strain 1103199
Klebsiella pneumoniae subsp. pneumoniae is a clinical isolate from BioMerieux that can be used as a quality control strain for chromID Oxa 48 medium. This strain is resistant to ertapenem, imipenem, and meropenem and the depositor claims that this strain is a carbapenemase producer (OXA-48).
Eurofins Discoverx FFAR1 (GPR40) Gq Calcium Signaling Cell-Based Assay Kit (CHO-K1)
The FFAR1 (GPR40) Gq Stable Cell Line Calcium Signaling Assay (CHO-K1) contains a stable clonal cell line used to measure FFAR1 (GPR40) (GPCR) activation via an increase in intracellular calcium. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ATCC B Lymphocyte Cell Line, Homo Sapiens, Burkitt's Lymphoma, BSL 2
This B lymphocyte cell line, isolated from a 41-year-old White male with Burkitt's lymphoma, is intended for immunology research. It expresses high levels of CD77 (approx. 90%) and exhibits sensitivity to Shiga Toxin (Stx). Epstein-Barr virus (EBV) has been detected in this cell line.
- Biosafety level: BSL 2
- Species: Homo sapiens
- Cell type: B lymphocyte, lymphoblast; morphology lymphoblast
- Disease: Burkitt's lymphoma
- Growth properties: Suspension
- Derived from lymphoma cells of a 41-year-old White male
- Antigen expression: CD10+, CD19+, CD20+, CD40+ (10%), CD45+, CD77+ (90%), CD34–
- Sensitive to Shiga Toxin (Stx)
- Epstein-Barr virus (EBV) detected
- Frozen product format for storage in vapor phase of liquid nitrogen
- Population doubling time: Approx. 24 hr.
Eurofins Discoverx PathHunter U2OS ACVR1B/BMPR2 Dimerization Cell Line, 2 vials. (93-1072C3)
The PathHunter ACVR1B/BMPR2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. activin A) induced dimerization of ACVR1B/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1B target protein fused to PK alongside BMPR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1B/BMPR2 , detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS PRα Protein Interaction Cell Line, 2 vials. (93-0852C3)
The PathHunter PRα Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. testosterone, norgestrel) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged PRα (progesterone receptor alpha) NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of PRα, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx PathHunter CHO-K1 CCR1 β-Arrestin Cell Line, 2 vials. (93-0254C2)
The PathHunter CCR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL3) based activation of CCR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR1 and EA-tagged β-Arrestin-2. Activation of CCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter EBI2 Total Internalization U2OS Cell Line, 2 vials. (93-0910C3)
The PathHunter EBI2 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of EBI2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged EBI2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EBI2 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 rCHRM4 β-Arrestin Cell Line, 2 vials. (93-0870C2)
The PathHunter rCHRM4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 HRH3 Gi Cell Line, 2 vials. (95-0103C2)
The cAMP Hunter HRH3 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HRH3, a naturally Gi/Go coupled GPCR. Upon activation of HRH3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 CCR3 β-Arrestin Cell Line, 2 vials. (93-0629C2)
The PathHunter CCR3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL13/MCP-4) based activation of CCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR3 and EA-tagged β-Arrestin-2. Activation of CCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 MRGPRX1 β-Arrestin Cell Line, 2 vials. (93-0919C2)
The PathHunter MRGPRX1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. BAM) based activation of MRGPRX1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX1 and EA-tagged β-Arrestin-2. Activation of MRGPRX1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.