General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 mGPR91 (SUCNR1) β-Arrestin Cell Pool, 2 vials. (93-0589P2)
The PathHunter mGPR91 (SUCNR1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. succinate) based activation of mGPR91 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGPR91 and EA-tagged β-Arrestin-2. Activation of mGPR91 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable cell pool is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS FOXO3 Nuclear Translocation Cell Line, 2 vials. (93-0876C3)
The PathHunter U2OS FOXO3 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the PI3 pathway. This cell line enables assessment of ligand (e.g. wortmannin) based pathway activation via detection of resulting FOXO3 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged FOXO3 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of FOXO3 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Montana Molecular Borealis Arrestin OTR Assay - 10mL
Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Oxytocin receptor (OTR) assay. Add the unmodified OTR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, OTR BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Borealis kits for several other receptors are also available, and larger-sized kits are available on request. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.
Eurofins Discoverx ChemiScreen P2Y4 Purinergic Receptor Stable Cell Line, 2 vials. (HTS211C)
The ChemiScreen P2Y4 Purinergic Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. ATP, UTP) based activation of P2RY4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses P2RY4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of P2RY4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter U2OS TGFBR1A/ACVR2A Dimerization Cell Line, 2 vials. (93-1012C3)
The PathHunter TGFBR1A/ACVR2A Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. GDF-11) induced dimerization of TGFBR1A/ACVR2A receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1A target protein fused to PK alongside ACVR2A target protein fused to EA. Upon ligand-induced dimerization of TGFBR1A/ACVR2A, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS SMO(W535L) MEM-EA Pharmacotrafficking Assay, 2 vials. (93-0991C3)
The PathHunter U2OS SMO(W535L) MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting SMO. This cell line enables assessment of chaperone-based stabilization of SMO(W535L) via detection of rescued membrane trafficking. The included cell line overexpresses ProLink-tagged mutant SMO(W535L), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant SMO, it is trafficked to the membrane, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 NPY2R β-Arrestin Cell Line, 2 vials. (93-0212C2)
The PathHunter NPY2R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. peptide YY) based activation of NPY2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPY2R and EA-tagged β-Arrestin-2. Activation of NPY2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mCXCR3 β-Arrestin Cell Line, 2 vials. (93-0553C2)
The PathHunter mCXCR3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL11/I-TAC) based activation of mCXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR3 and EA-tagged β-Arrestin-2. Activation of mCXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS mOPRM1 β-Arrestin Cell Line, 2 vials. (93-0524C3)
The PathHunter mOPRM1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. met 5-enkephalin) based activation of mOPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRM1 and EA-tagged β-Arrestin-2. Activation of mOPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Section - Arrhythmia, infarct: Heart, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Biochain Institute Inc Frozen Tissue Section - Human Diabetic Diseased Tissue: Kidney, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx PathHunter HEK 293 AVPR2 β-Arrestin Cell Line, 2 vials. (93-0187C1)
The PathHunter AVPR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR2 and EA-tagged β-Arrestin-2. Activation of AVPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS ACVR1B/BMPR2 Dimerization Cell Line, 2 vials. (93-1072C3)
The PathHunter ACVR1B/BMPR2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. activin A) induced dimerization of ACVR1B/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1B target protein fused to PK alongside BMPR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1B/BMPR2 , detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Vector Biolabs human CDK9 shRNA Adenovirus (Ad-h-CDK9-shRNA)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA