General Cell Lines and Cell Cultures
Genscript Corporation CHO-K1/DP1/G15 Stable Cell Li
DP1 is a G sub s /sub -coupled GPCR expressed in the colon eye platelets eosinophils that bind to PGD2 and PGE2 DP1 can inhibit platelet aggregation and also inhibit leukotriene B4 and superoxide anion (O2-) release from human neutrophils It can relax smooth muscle and regulate eosinophil apoptosis DP receptor knockout OVA-challenged mice exhibit reduced Th2 cytokine levels and lymphocyte accumulation in the lung In addition they failed to produce asthmatic responses
Genscript Corporation CHO-K1/Cyno HVEM Stable Cell
HVEM is found on the surface of various cell types including hematopoietic and non-hematopoietic cells and is expressed mainly in spleen thymus bone marrow lung and intestines It has been identified as a canonical TNF receptor signaling through the TNF receptor-associated factors (TRAFs) leading to NF B activation Furthermore in addition to acting as a signaling receptor in binding to Ig superfamily members it acts as a ligand for these inhibitory receptors Therefore bidirectional signaling is possible for the HVEM-mediated signaling network which can be involved in positive or negative immunological reactions under different contexts
GeneCopoeia NCI-H1299 Cas9 Stable Cell Line, 2 x 10^6 cells, hygromycin resistance, lung carcinoma, RPMI1640 base medium
NCI-H1299 Cas9 Stable Cell Line, 2 x 10^6 cells, hygromycin resistance, lung carcinoma, RPMI1640 base medium
Genscript Corporation CHO-K1/OX-40L Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human OX-40L on the surface The surface expression of OX-40L is validated by FACS analysis
Genscript Corporation CHO-K1/PAR4 Stable Cell Line
Protease-activated receptor (PAR)-4 is a member of a unique family of GPCRs The protease-activated receptors (PARs) that are activated by proteolytic cleavage of the N-terminal domain of the receptor reveal a tethered ligand The PAR family consists of 4 receptors PAR1 and PAR3 are activated by thrombin and PAR2 and PAR4 are activated by several serine proteases (Macfarlane et al 2001) PAR4 is a recently identified low-affinity thrombin receptor that plays a pathophysiological role in many types of tissues including the lung Mice lacking PAR4 are protected from mesenteric arteriole thrombosis indicating that PAR4 is a potential target for treatment of thrombosis in humans
ATCC Lactococcus cremoris
Lactococcus cremoris strain NCDO 607 is a whole-genome sequenced bacterial type strain that has applications in media testing and food testing.
ATCC Salmonella bongori; Strain 1224.72; genomic DNA
Genomic DNA isolated from Salmonella bongori strain 1224.72. This bacterial strain is also available as ATCC Catalog No. 43975™.
ATCC Penicillium rubens; NRRL 832
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC PBAD18KAN PLASMID IN E COLI
One of several tightly controlled expression vectors (ATCC 87393 - ATCC 87402) regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning sites and need for translation initiation sequences. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Plasmid contains bla (ampR) sequences surrounding the cmLR gene which could promote recombination if this plasmid is used in combination with other compatible ampR plasmids. Recombination can be avoided by the use of recA host strains, or it can be used to advantage to intentionally exchange markers among plasmids. The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 8 bp