General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells, 2 Vials of Cells. (HTS135RTA)
The Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. free fatty acids) based activation of FFAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR3 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen C5aR Anaphylotoxin Receptor Membrane Preparation, 1 vial. (HTS017M)
The ChemiScreen C5aR Anaphylotoxin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of C5AR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen MC3 Melanocortin Receptor Membrane Preparation, 1 vial. (HTS022M)
The ChemiScreen MC3 Melanocortin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MC3R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay MCH2 Melanin-Concentrating Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS026RTA)
The Ready-to-Assay MCH2 Melanin-concentrating Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MCH) based activation of MCHR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MCHR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay MCH1 Melanin-Concentrating Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS025RTA)
The Ready-to-Assay MCH1 Melanin-concentrating Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MCH) based activation of MCHR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MCHR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC SFT; Gliosarcoma; Human (Homo sapiens)
There is a Permit requirement: License form needed to be completed Material Transfer Agreement Addendum for Screening Application
The forms are available here: https://www.atcc.org/support/order-support/permits-and-restrictions
ATCC Salmonella enterica; subsp. enterica Cerro
Salmonella enterica subsp. enterica serovar Cerro was isolated in 2019 from water in Pennsylvania. It has applications in enteric, infectious, and zoonotic disease research.
ATCC pgsA-745; Ovary; Chinese Hamster (Cricetulus griseus)
There is a Permit requirement: License form needed to be completed Material Transfer Agreement Addendum for Screening Application
The forms are available here: https://www.atcc.org/support/order-support/permits-and-restrictions
ATCC Pfizer Pneumococcal polysaccharide powder Type 54 (d15B) 200mg
Purified pneumococcal polysaccharide type 15B is manufactured by Pfizer, Inc. for small quantity distribution by ATCC. The pneumococcal polysaccharide type 15B can be used to assay for type-specific antibodies, research immune responses, and study immunodeficiencies.
Cayman Chemical GLP-11-32bulfrogtrIf 10mg
An endogenous incretin hormone; increases intracellular cAMP levels using HEK293 cells expressing human GLP-1R (EC50 = 0.051 nM); reduces blood glucose levels in an OGTT in fasted mice at 30 mg/kg
Apexbio Technology LLC NFAT Inhibitor 249537-73-3 1mg
NFAT Inhibitor (CAS 249537-73-3) is a selective small molecule that blocks the calcineurin-dependent dephosphorylation of the nuclear factor of activated T cells (NFAT) thereby preventing its activation Unlike general calcineurin inhibitors this compound does not interfere with other calcineurin-mediated signaling pathways By inhibiting NFAT activation it reduces the expression of NFAT-dependent cytokine genes in T cells NFAT Inhibitor is valuable for studying the specific roles of NFAT signaling in immune cell function and for dissecting calcineurin-related pathways in immunological and cellular research
Eurofins Discoverx Ready-to-Assay C3a Anaphylotoxin Receptor Frozen Cells, 2 Vials of Cells. (HTS016RTA)
The Ready-to-Assay C3a Anaphylotoxin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. C3a) based activation of C3AR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses C3AR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of C3AR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CB2 Cannabinoid Receptor Stable Cell Line, 2 vials. (HTS020C)
The ChemiScreen CB2 Cannabinoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. cannabinoids, CP 55,940) based activation of CNR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CNR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CNR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC ROUS ASSOCIATED VIRUS - A, Strain RAV-1 Retrovirus, Avian
Rous associated virus-A (Avian leukosis-A) strain RAV-1 is propagated in chicken embryo fibroblasts. This strain was isolated from a chicken fibrosarcoma. RAV-1 is one of the helper viruses for the Bryan high-titer RSV.