General Cell Lines and Cell Cultures
![Biolegend Alexa Fluor 488 anti-α Smooth Muscle Actin [1A4]; Isotype: Mouse IgG2a, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICC; Size: 25 μg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502723719.png-250.jpg)
Biolegend Alexa Fluor 488 anti-α Smooth Muscle Actin [1A4]; Isotype: Mouse IgG2a, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICC; Size: 25 μg
Alexa Fluor 488 anti-α Smooth Muscle Actin [1A4]; Isotype: Mouse IgG2a, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICC; Size: 25 μg
Angio Proteomie RFP Expressing Human Umbilical Vein Endothelial Cells (RFP-HUVECs), Red Fluorescent Protein (RFP), 5 x 10^5 cells/vial, Passage 3, Zeocin resistant, >18 minimum population doublings
RFP Expressing Human Umbilical Vein Endothelial Cells (RFP-HUVECs), Red Fluorescent Protein (RFP), 5 x 10^5 cells/vial, Passage 3, Zeocin resistant, >18 minimum population doublings
Enzo Life Sciences LEADING LIGHT Wnt Reporter Cell Line (1 ml)
Wnt reporter cell line for discovering Wnt pathway modulators. The LEADING LIGHT. .The assay has been used successfully in different assay formats (including HTS applications) to identify several distinct categories of small molecule compounds that modulate the Wnt signaling pathway..Note: the complete LEADING LIGHT Wnt Reporter Assay Starter Kit (Prod. No. ENZ-61001) is available. Alternative name: Wnt reporter cell line. Shipping: Shipped on Dry Ice. Long Term Storage: -80°C
Biolegend PE anti-mouse CD24 Recombinant
PE anti-mouse CD24 Recombinant [QA20A91]; Isotype: Mouse IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 100 ug
Brainxell Inc Spinal Astrocytes (Human iPSC-Derived, Male Line, 1M)
Spinal astrocytes derived from iPSC line WC-30, male. Contents: 1 vial of ≥1x10^6 viable cryopreserved cells. Marker Expression and Morphology: Spinal Astrocytes (BX-0650) express key astrocyte markers GFAP (green) at >80% and exhibit mature process-bearing morphologies (shown here with a DAPI stain). Labelling by the spinal-specific marker HOXB4 (red) with a Hoescht stain highlights the regional specificity of the Spinal Astrocytes. Function: Spinal Astrocytes promote mature motor neuron activity. Co-culturing motor neurons with spinal astrocytes promotes maximal calcium influx within 10 days. Co-culture spinal astrocytes with motor neurons also accelerates the development of activity recorded by multi-electrode array. Related diseases: Amyotrophic Lateral Sclerosis (ALS), Spinal muscular atrophy (SMA), & Spinal Cord Injury.
ATCC NRAS mutant-A375; CRISPR/Cas9; Modified Cell line; Human (Homo sapiens), Isogenic
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
Abeomics Sre Reporter Hek293 Cell Line
The SRE Leeporter Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response element (SRE). The SRE reporter cell line is designed to monitor MAPK/ERK activity and can be used for studying GPCR-linked MAPK/ERK signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the MAPK/ERK signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay. Cell line application: (1) Monitor the GPCR-linked MAPK/ERK signaling pathway, and (2) Screen for activators or inhibitors of the GPCR-linked MAPK/ERK signaling pathway. .
ATCC Quantitative Synthetic; Human immunodeficiency; virus 1 RNA
Quantitative Synthetic Human immunodeficiency virus 1 RNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. Preparation includes fragments from 5’ LTR, gag gene, pol gene (including protease, reverse transcriptase and integrase regions), tat gene, rev gene, and nef gene.
Eurofins Discoverx Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells, 2 Vials of Cells. (HTS139RTA)
The Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses SSTR5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR5, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay PAC1-long Receptor Frozen Cells, 2 Vials of Cells. (HTS114RTA)
The Ready-to-Assay PAC1-long Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADCYAP1R1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADCYAP1R1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS099RTA)
The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiBrite EP3 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS092L)
The ChemiBrite EP3 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ILEX LIFE SCIENCES LLC EMEM FIBROBLASTS 6 X 500 ML
PAN-Biotech EMEM Cell Culture Medium for Fibroblasts, w/o: L-Glutamine, w: 2.2 g/L NaHCO3 (6 x 500 ml). BICARBONATE: With 2.2 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: EMEM Fibroblasts is a cell culture medium based on MEM Eagle (Eagle's Minimum Essential Medium) which was in turn developed from BME (Basal Medium Eagle). EMEM Fibroblasts is optimized specifically for the cultivation of fibroblasts. EMEM Fibroblasts is based on EMEM supplemented with additional or higher amount of amino acids and vitamins and is thus optimized for improved growth of fibroblasts. For cultivation of fibroblasts, this medium must be supplemented with 10% FBS before use.
Eurofins Discoverx ChemiScreen CysLT2 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS088C)
The ChemiScreen CysLT2 Leukotriene Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene) based activation of CYSLTR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CYSLTR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CYSLTR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.