General Cell Lines and Cell Cultures
Lonza Walkersville Transfection Transfection Kit 1/PK
Electroporation-based methods have so far required cells to be in suspension for transfection However the 4D-Nucleofector Y Unit allows direct Nucleofection of cells in adherence Cells which typically grow in adherence in cell culture can be kept and transfected by Nucleofection in their physiological state The AD2 Primary Cell 4D-Nucleofector Y Kit is one of our two kits that are suited for electroporation of primary cells in adherence when working with the 4D-Nucleofector Y Unit The cells are cultured in the 24-well culture plates provided with the kit and for the transfection step a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific Nucleofector Solution mixed with the DNA or RNA substrate
Eurofins Discoverx InCELL Hunter U2OS Bcl2L1-Bim Protein Binding Cell Line, 2 vials. (96-0037C3)
The InCELL Hunter U2OS Bcl2L1-Bim Protein Binding Cell Line provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bim binding via detection of Bim levels. This product uses EFC technology. The cell line included overexpresses Bcl2L1 target in an untagged form and Bim binding partner fused to ProLabel. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bim is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Abcam Tandospirone hydrochloride (Metanopirone), 5-HT1A partial agonist, 50MG
MW 419.9 Da, Purity >99%. Potent, selective 5-HT1A partial agonist (Ki = 27 nM). Decreases the firing rate of 5-HT neurons of the dorsal raphe. Shows anxiolytic and antidepressant effects *in vivo.* Orally active.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter CHO-K1 GPR173 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0380C2A)
The PathHunter GPR173 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR173 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR173 and EA-tagged β-Arrestin-2. Activation of GPR173 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mEDNRB β-Arrestin Cell Line, 2 vials. (93-0602C2)
The PathHunter mEDNRB cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. endothelin-3) based activation of mEDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRB and EA-tagged β-Arrestin-2. Activation of mEDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 OPRM1 Gi Cell Line, 2 vials. (95-0107C2)
The cAMP Hunter OPRM1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. met-enkephalin, morphine) based activation of OPRM1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRM1, a naturally Gi/Go coupled GPCR. Upon activation of OPRM1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Section - Human Tumor: Ovary, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Genscript Corporation CHO-K1/B7-H3 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human CD276 (B7-H3) on the surface The surface expression of B7-H3 is validated by FACS analysis This cell line is recommended for cell-based binding assay to screen antibodies against B7-H3 or to measure binding affinity between B7-H3 and anti-B7-H3 antibodies
ATCC Klebsiella pneumoniae; 6 x 0.3mL
Klebsiella pneumoniae subsp. pneumoniae strain PCI 602 (ATCC 10031-MINI-PACK) consists of 6 ready-to-use vials of ATCC 10031 frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping. This product has applications in respiratory and infectious disease research, inhibition testing, and quality control.
Eurofins Discoverx Ready-to-Assay LH Glycoprotein Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS233LRTA)
The Ready-to-Assay LH Glycoprotein Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. luteinizing hormone, hCG) based activation of LHCGR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LHCGR alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of LHCGR, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC BC-1; Lymphoma; Human (Homo sapiens)
There is a Permit requirement: License form needed to be completed Material Transfer Agreement Addendum for Screening Application
The forms are available here: https://www.atcc.org/support/order-support/permits-and-restrictions
Cell Applications Inc Human Brachiocephalic Artery Endothelial Cells Total Kit
Human Brachiocephalic Artery Endothelial Cells Total Kit:(3012-05a) Cryopreserved (HBcAEC), 1 vial(212-500) MesoEndo Cells Growth Media, 500ml(090K) Subculture Reagent Kit
Cell Applications Inc Bovine Coronary Artery Endothelial Cells Total Kit
Bovine Coronary Artery Endothelial Cells Total Kit:(B300-05) Cryopreserved (BCAEC) 1 vial(B211-500) Bovine Endothelial Cells Growth Media, 500ml(090K) Subculture Reagent Kit
Cell Applications Inc Porcine Aortic Smooth Muscle Cells Total Kit
Porcine Aortic Smooth Muscle Cells Total Kit:(P354-05) Cryopreserved (PAOSMC), 1 vial(P311-500) Porcine Smooth Muscle Cell Growth Media, 500ml(090K) Subculture Reagent Kit
Cell Applications Inc Human Femoral Artery Endothelial Cells Total Kit
Human Femoral Artery Endothelial Cells Total Kit:(3016-05a) Cryopreserved (HFAEC) 1 vial, adult(211-500) Endothelial Cells Growth Media, 500ml(090K) Subculture Reagent Kit